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巨噬细胞炎性蛋白1α和载脂蛋白E基因多态性与炎症性肠病易感性的关系
作者姓名:Liang WD  Yang JF  Yan J  Jin J  Li KS  Li JS  Bi YT
作者单位:1. 温州医学院生命科学院生物系,325035
2. 温州医学院第二附属医院消化科
3. 温州市第二人民医院消化内科
4. 广东医学院临床医学研究中心
5. 温州医学院基础医学院生理教研室
摘    要:目的 探讨巨噬细胞炎性蛋白1α(MIP-1α)基因和载脂蛋白E(ApoE)基因多态性是否与炎症性肠病(IBD)的发生相关,并探讨这两个基因之间的相互作用.方法 采用聚合酶链反应-限制性片段长度多态性方法,在183例IBD患者包括142例溃疡性结肠炎(UC)患者和41例克罗恩病(CD)患者]以及160名健康对照者中检测MIP-lα和ApoE等位基因和基因型的频率.结果 携带MIP-1α-906(TA)6/(TA)6基因型或者APOE484等位基因可以增加UC的发病风险,UC患者组MIP-1α-906位点(TA)6/(TA)6基因型频率为50%,显著高于正常对照组中的34%(OR=1.909,95%C/=1.204~3.028);UC患者ApoE ε4+基因型频率为40.8%,显著高于正常对照组中的22.5%(OR=2.379,95%CI=1.451~3.896).同时携带MIP-1α-906(TA)6/(TA)6和APOE4ε4的个体发生UC的风险更大(P<0.01,OR=3.288,95%17/=1.777~6.084).而CD组与对照组间的MIP-lα和ApoE基因多态频率差异无统计学意义.结论 MIP-lot基因-906(TA)6/(TA)6多态性和APOFAε4基因多态性可能是UC发病的遗传危险因素;MIP-1α基因多态性与ApoE基因多态性之间有协同作用,ApoEε4等位基因与MIP-1α-906(TA)6/(TA)6基因型同时存在时,患UC的危险性显著提高.
Abstract:
Objective To investigate whether the macrophage inflammatory protein 1 alpha(MIP-1α)and apolipoprotein E(ApoE)gene polymorphisms,either alone or in combination,affect the susceptibility to inflammatory bowel disease(IBD).Methods Genomic DNA of IBD patients with Crohn's disease(CD,n=41)and with ulcerative colitis(UC,n=142)and healthy controls(n=160)was extracted and genotyped for the MIP-1α and ApoE gene polymorphisms by restriction fragment length polymorphism assay.Results MIP-1α-906(TA)6/(TA)6 homozygotes had a significantly elevated risk of UC(OR=1.909,95%CI=1.204-3.028).The carriers of APOF4ε4 were at a significantly higher risk for UC with OR of 2.379(95%CI=1.451-3.896).And a combination of these two loci,MIP-1α-906(TA)6/(TA)6/APOE4ε4 were strongly associated with a higher risk of UC(OR=3.288;95%CI=1.777-6.084).Conclusion The polymorphisms of MIP-1α-906(TA)6/(TA)6 and ApoE are probably independent genetic risk factors for UC.And the coexistence of both may exert an additive effect on the UC risks.

关 键 词:结肠炎  溃疡性  Crohn病  MIP-1α基因  载脂蛋白类  基因  多态性

Association of combined polymorphisms in MIP-1α and ApoE genes with the susceptibility of inflammatory bowel disease
Liang WD,Yang JF,Yan J,Jin J,Li KS,Li JS,Bi YT.Association of combined polymorphisms in MIP-1α and ApoE genes with the susceptibility of inflammatory bowel disease[J].National Medical Journal of China,2011,91(18):1250-1253.
Authors:Liang Wan-Dong  Yang Ji-Feng  Yan Jun  Jin Jie  Li Ke-Shen  Li Jin-Song  Bi Yun-Tian
Institution:School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China.
Abstract:Objective To investigate whether the macrophage inflammatory protein 1 alpha(MIP-1α)and apolipoprotein E(ApoE)gene polymorphisms,either alone or in combination,affect the susceptibility to inflammatory bowel disease(IBD).Methods Genomic DNA of IBD patients with Crohn's disease(CD,n=41)and with ulcerative colitis(UC,n=142)and healthy controls(n=160)was extracted and genotyped for the MIP-1α and ApoE gene polymorphisms by restriction fragment length polymorphism assay.Results MIP-1α-906(TA)6/(TA)6 homozygotes had a significantly elevated risk of UC(OR=1.909,95%CI=1.204-3.028).The carriers of APOF4ε4 were at a significantly higher risk for UC with OR of 2.379(95%CI=1.451-3.896).And a combination of these two loci,MIP-1α-906(TA)6/(TA)6/APOE4ε4 were strongly associated with a higher risk of UC(OR=3.288;95%CI=1.777-6.084).Conclusion The polymorphisms of MIP-1α-906(TA)6/(TA)6 and ApoE are probably independent genetic risk factors for UC.And the coexistence of both may exert an additive effect on the UC risks.
Keywords:Colitis  ulcerative  Crohn disease  MIP-1 alpha genes  Apolipoproteins  Gene  polymorphism
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