| 过氧亚硝基阴离子参与介导内毒素所致急性肺损伤的研究 |
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| 引用本文： | 谷振勇,凌亦凌,丛斌.过氧亚硝基阴离子参与介导内毒素所致急性肺损伤的研究[J].中华医学杂志,2000,80(1):58-61. |
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| 作者姓名： | 谷振勇 凌亦凌 丛斌 |
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| 作者单位： | ：谷振勇（050017
石家庄市，河北医科大学病理生理教研室）;凌亦凌（050017
石家庄市，河北医科大学病理生理教研室）;丛斌（050017 石家庄市，河北医科大学病理生理教研室）;朱铁年（050017
石家庄市，河北医科大学病理生理教研室） |
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| 基金项目： | ：国家自然科学基金资助项目(39870317) |
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| 摘 要： | 目的 探讨过氧亚硝基阴离子（ＯＮＯＯ＾－）在脂多糖（ＬＰＳ）所致急性肺损伤中的作用。方法 用免疫组织化学技术检测内毒素休克时ＯＮＯＯ＾－生成的标志物硝基酷氨酸在大鼠肺脏内的定位与分布。将不同剂量的外源性ＯＮＯＯ＾－经气管快速推注入健康大鼠肺脏，定量检测肺系数、肺湿干重比、肺水含量和伊文思蓝含量，光镜观察单层蓝标记肺微血管数量及病理变化。结果 ＬＰＳ静脉注射２ｈ和６ｈ后，肺脏有明显损伤及硝基酷氨酸产
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| 关 键 词： | 一氧化氮 脂多糖类 肺损伤 内毒素休克 并发症 |
| 修稿时间： | 1999-02-04 |
Peroxynitrite mediated acute lung injury induced by lipopolysaccharides in rats |
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| GUZhenyong,LING Yiling,CONGBin,etal.Peroxynitrite mediated acute lung injury induced by lipopolysaccharides in rats[J].National Medical Journal of China,2000,80(1):58-61. |
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| Authors: | GUZhenyong LING Yiling CONGBin etal |
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| Institution: | Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China. |
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| Abstract: | Objective Toexploretherolesofperoxynitrite(ONOO-)inacutelunginjuryinducedby lipopolysaccharides (LPS) Methods Nitrotyrosine (NT) ,aspecificmarkerofinvivoONOO-production ,was detectedwithimmunohistochemicaltechniqueinratlungduringendotoxinshock (LPS 5mg/kgiv) Lung coefficient(LC) ,lungwet/dryweightratio (W/D) ,thecontentsofwaterandEvansblue(EB)inlungswere measuredafterintratrachealinsufflationofONOO-withdifferentdoses Monastralblue (MB) labelledblood vesselsandpathologicalchangesinlungswereexaminedunderalightmicroscope Results After 2or 6hoursof LPSintravenousadministration ,severelunginjuryandpositivesignalsofNToccurredwiththelatterlocatedinthe pulmonarymacrophages,endothelia ,subendothelialandmuscularlayersofthepulmonaryartery Administrationof exogenousONOO-causeddose dependentincreaseinLC ,W /D ,waterandEBcontentsandinthenumberofMB labelledbloodvesselsinratlungs,togetherwithsignificantpathologicalalterationssuchasdiffusealveolar collapse ,capillarycongestionintheseptaandfocalhemorrhage Conclusion Intheearlystageofendotoxin shock,severelunginjuryandONOO-productionsimultaneouslyexist,andexogenousONOO-couldinduce significantdysfunctionofmicrovascularendothelialbarrierandlunginjury,indicatingthatONOO-generationin vivomaybeinvolvedinthepathogenesisofacutelunginjury |
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| Keywords: | Nitricoxide Lipopolysaccharide Lung |
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