肥胖及2型糖尿病患者脂肪组织视黄醇结合蛋白4 mRNA的表达及调控

目的 研究肥胖及2型糖尿病患者皮下和腹内脂肪组织视黄醇结合蛋白4(RBP4)mRNA的表达差异,并探讨影响其表达调控的因素.方法 选取2007年1-6月因非代谢性疾病而接受腹部择期手术的正常体重正常糖调节、单纯肥胖、2型糖尿病患者各9例,取皮下和腹内脂肪组织,用RT-PCR法检测RBP4 mRNA的表达,并在体外对正常体重正常糖调节者腹内脂肪组织分别与一定浓度的胰岛素、地塞米松、棕榈酸、肿瘤坏死因子-α、吡咯列酮共培养,用RT-PCR法检测药物对腹内脂肪组织RBP4 mRNA表达的变化.结果 肥胖和2型糖尿病患者腹内脂肪RBP4 mRNA的表达均显著高于正常体重正常糖调节者(分别为2.10±1.84和1.54±0.46比0.75±0.28,P＜0.01和P＜0.05),并明显高于相应的皮下脂肪组织;3组人群间皮下脂肪组织RBP4 mRNA的表达差异无统计学意义(1.05±0.15比0.99±0.14比1.13±0.07,P＞0.05);药物胰岛素、地塞米松、吡咯列酮、棕榈酸能显著上调RBP4 mRNA的表达,与对照组相比,分别上升了2.13、0.84、2.04、4.88倍;肿瘤坏死因子-α显著下调RBP4 mRNA的表达,较对照组下降了38%.结论 肥胖和2型糖尿病患者腹内脂肪组织RBP4 mRNA的表达显著上升,正常体重正常糖调节者腹内脂肪组织RBP4 mRNA的表达受胰岛素、地塞米松等多种参与糖脂代谢及胰岛素抵抗因素的调控.

Expression and regulation of retinol binding protein 4 mRNA in human adipose tissue in obese and type 2 diabetics

Objective To study the RBP4 mRNA expression between subcutaneous and visceral adipose tissue in obese and type 2 diabetic patients and to investigate the factors that influence RBP4 mRNA expression in Human visceral adipose tissue. Methods 9 individuals with normal weight normal glucose regulation subjects, 9 obesity subjects and 9 type 2 diabetes subjects were enrolled. All of the subjects were prepared to undergone an operation because of nondiabetes disease. Subcutaneous and visceral adipose tissue were taken out as soon as cultured. RT-PCR and Real-time PCR were used to assay the relative expression of RBP4 mRNA. Results RBP4 mRNA level in visceral adipose tissue of obesity group was (2. 10 ± 1.84),and that of type 2 diabetes group was ( 1.54 ± 0. 46 ), both were significantly higher than that in normal weight normal glucose group ( 0. 75 ± 0. 28, P ＜ 0. 01 , P ＜ 0. 05 ). RBP4 mRNA level in subcutaneous adipose tissue of the three groups were ( 1.05 ±0. 15 vs 0. 99 ±0. 14 vs 1.13 ±0. 07), no difference among them(P ＞ 0. 05 ). Insulin, dexamethasone, pioglitazone, free fatty acids can significantly increase RBP4 mRNA expression, compared with the control group, respectively, have an increase of 2. 13 times, 0. 84times, 2. 04 times, 4. 88 times; however, tumor necrosis factor-αt can significantly lower RBP4 mRNA level, compared with the control group decreased by 38%. Conclusion RBP4 mRNA expression in visceral adipose tissue were significantly higher in obesity and type 2 diabetes subjects. In vitro system, RBP4 gene expression in visceral adipose tissue of normal weight normal glucose subjects was regulated by insulin,dexamethasone, pioglitazone, palmitic acid and TNF-α, such factors were also participated in the pathophysiological process of insulin resistance and type 2 diabetes.