首页 | 本学科首页   官方微博 | 高级检索  
检索        

X型胶原基因在特发性脊柱侧凸患者顶椎椎间盘内表达的初步研究
作者姓名:He HL  Wu ZH  Zhang JG  Wang YP  Zhou Y  Xu YQ  Yuan JG  Qiu GX
作者单位:1. 第二军医大学附属长征医院骨科
2. 100730,中国医学科学院,中国协和医科大学,北京协和医院骨科
3. 中国医学科学院生物化学系国家分子生物学重点实验室
基金项目:国家自然科学基金资助项目 (3 0 3 0 70 765 )
摘    要:目的 研究X型胶原基因在椎间盘内的分布及其在特发性脊柱侧凸发病中的作用。方法 收集 14例特发性脊柱侧凸患者顶椎及中间椎椎间盘共 2 8个 ,13例已知病因的脊柱侧凸患者顶椎及中间椎椎间盘共 2 6个为对照。 3例突然死亡的正常青少年椎间盘共 6个作为正常对照。采用免疫组织化学技术和RNA探针原位杂交技术研究X型胶原基因mRNA在椎间盘内不同部位的分布。终板软骨内X型胶原原位杂交图像输入图像分析系统 ,比较X型胶原mRNA含量在不同部位的分布。结果 X型胶原主要分布于肥大的软骨细胞周围 ,mRNA分布于终板软骨内肥大的软骨细胞。在少量侧凸患者髓核内也可见其少量阳性表达 ,但与病因无关。特发性侧凸组顶椎及中间椎 ,已知病因组顶椎及中间椎X型胶原mRNA含量均高于正常对照组 (P <0 0 5 ) ,而特发性侧凸组与已知病因组间差异无显著意义 (P >0 0 5 )。结论 X型胶原主要分布于终板软骨内肥大软骨细胞 ,髓核内部分类软骨细胞也具有分泌作用。侧凸患者X型胶原表达量显著增高主要由于长期应力引起了软骨的早期钙化。X型胶原在侧凸患者髓核内的表达可能是髓核内类软骨细胞异常应力下分泌的结果

关 键 词:X型胶原  患者  椎间盘  软骨细胞  特发性脊柱侧凸  终板  肥大  原基  分布  病因

Primary study on collagen X gene expression in the apical disc of idiopathic scoliosis
He HL,Wu ZH,Zhang JG,Wang YP,Zhou Y,Xu YQ,Yuan JG,Qiu GX.Primary study on collagen X gene expression in the apical disc of idiopathic scoliosis[J].National Medical Journal of China,2004,84(20):1681-1685.
Authors:He Hai-Long  Wu Zhi-Hong  Zhang Jian-Guo  Wang Yi-Peng  Zhou Yan  Xu Ya-Qin  Yuan Jian-Gang  Qiu Gui-Xing
Institution:Orthopaedics Department of Peking Union Medical College Hospital, Beijing 100730, China.
Abstract:Objective To study the distribution of collagen X in the intervertebral disc and determine its role in the pathogeny of idiopathic scoliosis (IS) Methods The data included apical disc and intermediate disc from 14 cases of AIS, 26 discs from 13 cases of scoliosis of confirmed pathogeny which included 10 cases of congenital scoliosis and neurofibromatosis scoliosis (CS group) Six discs were obtained from 3 cases of sudden death of normal young man served as controls The distribution of collagen X in the apical disc of IS was examined by immunohistochemistry and in situ hybridization(ISH) with RNA probe The figure of collagen X hybridization in the endplate cartilage was input to the figure analysis system The mRNA content of collagen X was compared between every 2 groups by SPSS software Results Collagen X was mainly distributed around the hypertrophic chondrocyte in the endplate cartilage Its mRNA was expressed in the hypertrophic chondrocyte Positive signal was found in the nuclei of several scoliosis patients, which was not related to the pathogeny The collagen X mRNA contents of the apical disc and intermediated disc of the IS group and of the CS group were significantly higher than those of the normal group ( P <0.05). There was no difference between the IS and CS groups Conclusions Collagen X is mainly distributed around the hypertrophic chondrocyte in the endplate cartilage Its can also be secreted by the chondrocyte like cells in the nucleus under special condition Higher expression of collagn X gene in scoliosis patients may be the effects of long term abnormal stress which causes calcification of endplate cartilage Collagen X expressed in the nucleus may be the result of secretion of chondrocyte like cells in the disc under abnormal mechanical condition
Keywords:Collagen  Genes  Scoliosis
本文献已被 CNKI 万方数据 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号