人鼻咽癌自发性高淋巴道转移模型的建立及生物学特性研究

目的在裸鼠体内建立人鼻咽癌自发性高淋巴道转移模型,并研究该癌细胞的有关生物学特性。方法将鼻咽癌单克隆细胞株（ＣＮＥ２ＺＨ５）移植于裸鼠皮下,待裸鼠发生转移时,取其淋巴结转移灶癌细胞再次移植裸鼠皮下传代,重复传９代后,观察各代裸鼠的转移率和转移途径;各代癌细胞体外增殖能力、癌细胞生长因子和受体表达的变化;裸鼠体内移植瘤增殖指数及瘤重和体积倍增时间的变化。结果癌细胞在裸鼠体内传９代后,各代裸鼠的淋巴结转移率一直稳定在８５％以上,且转移途径较为单一;各代癌细胞的体内外增殖能力不断增强。结论建立了人鼻咽癌自发性高淋巴道转移模型;鼻咽癌细胞在裸鼠体内演进中转移能力和增殖能力均不断增强,两者关系密切

A model of nasopharyngeal carcinoma with spontaneous highly lymphatic metastasis in nude mice and its biological characteristics

Objectives To establish a model of nasopharyngeal carcinoma with spontaneous highly lymphatic metastasis in nude mice and to study its biological characteristics. Methods The clone cells of nasopharyngeal carcinoma(NPC), CNE 2Z H5, were inoculated into nude mice.The cancer cells of lymph node metastatic foci were transplanted into nude mice again when the metastasis of nude mice were observed. After repetition of this procedure for 9 cycles, the metastatic rate and the metastatic paths were observed in nude mice of every passage; The changes were also observed in proliferation ability, expression of growth factor and its receptor in vitro, proliferation index(PI), and tumor weight and doubling time of the transplantable tumor in every passage in vivo. Results The lymphatic metastatic rates were higher than 85%.The metastatic paths were single, the proliferation ability was increased in cancer cells of every passage in vivo and vitro after passaging cancer cells of lymphatic metastatic foci for 9 cycles in nude mice. Conclusions A model of nasopharyngeal carcinoma with spontaneous highly lymphatic metastasis has been established in nude mice, and the possiblites of metastasis and proliferation are increased after progression of NPC cells in nude mice. There is a close relationship between the metastasis and proliferation in NPC.