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大鼠臂丛神经损伤后骨骼肌萎缩时肌细胞凋亡的研究
引用本文:田涛,吴朝晖.大鼠臂丛神经损伤后骨骼肌萎缩时肌细胞凋亡的研究[J].中华医学杂志,2000,80(7):530-533.
作者姓名:田涛  吴朝晖
作者单位:田涛(200032 上海医科大学病理生理学教研室)
基金项目:美国中华医学基金资助项目!(95619)
摘    要:目的 研究大鼠臂丛神经损伤后骨骼肌萎缩与细胞凋亡的关系以及肌细胞凋亡的基因调控机制。方法 应用臂丛神经损伤后骨骼肌萎缩的大鼠模型,采用流式细胞仪PI法、PI和AnnexinV双标记法、原位末端标记、DNA凝胶电泳和电镜等多种方法有骼肌萎缩与细胞凋亡的关系。并用免疫组织化学和Norgtherm-Blot方法检测凋亡相关基因Fas、FADD、Caspase-8、Bcl-2、P53、c-myc的表达。结

关 键 词:骨骼肌萎缩  臂丛神经损伤  肌细胞凋亡
修稿时间:1999-09-07

Apoptosis in atrophic skeletal muscle induced by brachial plexus injury in rats
TIAN Tao,WU Zhaohui,JIN Huiming.Apoptosis in atrophic skeletal muscle induced by brachial plexus injury in rats[J].National Medical Journal of China,2000,80(7):530-533.
Authors:TIAN Tao  WU Zhaohui  JIN Huiming
Institution:Department of Pathophysiology, Shanghai Medical University, Shanghai 200032, China.
Abstract:OBJECTIVE: To study the role of apoptosis and the expression of apoptosis-associated genes in skeletal muscle atrophy induced by brachial plexus injury in rats. METHODS: Rat models of skeletal muscle atrophy were established by cutting off brachial plexus of one upper limb. Apoptosis of muscular cells was investigated by TUNEL, flowcytometry, DNA electrophoresis and electromicroscopic observation. The apoptosis associated genes such as Fas, FADD, Caspase 8, c-myc, P53 and Bcl-2 were detected by immunohistochemical method (ABC) and Northern-blot. RESULTS: It was found with TUNEL and flowcytometry that the percentage of apoptotic muscle cell rose obviously in atrophic skeletal muscle (P < 0.05). DNA laddering could be seen in DNA gel electrophoresis of atrophic muscle after brachial injury. Morphologic changes of early stage in apoptotic cell could be seen under eletcromicroscope, such as the aggregation of chromosome, the expansion of nucleic cistern and the contraction of nucleus. Fas, FADD and Caspase genes were expressed highly and Bcl-2 gene was expressed lowly with immunohistochemical method in atrophic muscle. The results were all significantly different with that of the controls (P < 0.01). But the expression changes in P53 and c-myc genes were not obvious. The result of Northern-blot indicated that the mRNA of Fas gene rose and that of Bcl-2 gene decreased obviously (P < 0.01) in atrophic muscle induced by brachial plexus injury. CONCLUSION: There are much more apoptotic cells in atrophic muscle induced by brachial plexus injury, and apoptosis plays an important role in its pathogenesis. The apoptotic signal maybe transmitted through Fas-->FADD-->Caspase 8, and the decrease in Bcl-2 gene expression aggravates the process.
Keywords:Skeletal muscle atrophy  Denervation  Apoptosis
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