首页 | 本学科首页   官方微博 | 高级检索  
检索        

微RNA-21在乳腺浸润性导管癌中的表达及与磷酸酶张力蛋白基因的相关分析
引用本文:黄关立,张筱骅,郭贵龙,黄卡特,杨开颜,胡孝渠.微RNA-21在乳腺浸润性导管癌中的表达及与磷酸酶张力蛋白基因的相关分析[J].中华医学杂志,2008,88(40).
作者姓名:黄关立  张筱骅  郭贵龙  黄卡特  杨开颜  胡孝渠
作者单位:1. 温州医学院附属第一医院肿瘤外科,温州,325000
2. 温州医学院附属第一医院病理科,温州,325000
摘    要:目的 探讨微RNA-21(mir-21)和第10染色体同源丢失性磷酸酶张力蛋白基因(PTEN)在乳腺浸润性导管癌中的表达情况及临床意义.方法 采用茎环实时荧光逆转录聚合酶链反应(realtime RT-PCR)检测40例乳腺浸润性导管癌及其对应的癌旁正常乳腺组织中mir-21表达,免疫组化Envision法检测相应癌组织中PTEN表达,分析mir-21表达和PTEN表达的相关性及其与临床病理特征的关系.结果 茎环realtime RT-PCR检测mir-21表达的敏感性和特异性良好;mir-21在乳腺癌组织中的表达显著高于正常组织(P<0.001);PTEN在癌组织中的低表达率为45%(18/22).PTEN低表达组mir-21的表达显著高于PTEN高表达组(P=0.013).mir-21的高表达还和较高的TNM分期、淋巴结转移及高增殖指数有关(P值分别为0.021,0.010和0.030).结论 mir-21在乳腺浸润性导管癌中的表达升高,可能和乳腺癌的增殖、侵袭转移有关;在乳腺癌中PTEN可能为其靶基因之一.

关 键 词:乳腺肿瘤  微RNA  微RNA-21  PTEN磷酸水解酶

Expression of microRNA-21 in invasive ductal carcinoma of the breast and its association with phosphatase and teusin homolog deleted from chromosome expression and clinicopathologic features
HUANG Guan-li,ZHANG Xiao-hua,GUO Gui-long,HUANG Ka-te,YANG Kai-yan,HU Xiao-qu.Expression of microRNA-21 in invasive ductal carcinoma of the breast and its association with phosphatase and teusin homolog deleted from chromosome expression and clinicopathologic features[J].National Medical Journal of China,2008,88(40).
Authors:HUANG Guan-li  ZHANG Xiao-hua  GUO Gui-long  HUANG Ka-te  YANG Kai-yan  HU Xiao-qu
Abstract:Objective To investigate the expression of microRNA-21 (mir-21) in invasive ductal carcinoma (IDC) of the breast and its association with phosphatase and tensin homologue deleted from chromosome (PTEN)-I0 protein expression and the clinicopathologic features of IDC. Methods Specimens of IDC and normal tissues more than 5 cm away from the tumor tissues were collected from 40 IDC patients, all female, aged 53 (35 -77). Stem-loop real-time RT-PCR was used to examine the mir-21 expression. Immunohistochemistry was used to examine the PTEN protein expression in the tumor tissue. The association of mir-21 expression with the PTEN expression and the clinicopathologic features of the breast IDC were analyzed. Results Compared with the nontumor control samples, the median (M) of relative expression of mir-21 (2-△△Ct) was 5. 770 (25th-75th percentile, 3. 605-7. 255) in the tumor samples, significantly higher than that of the nontumor control samples (set at 1. 000, P < 0. 001). Reduced PTEN protein expression was seen in 45% (18/22) of all cases. The expression of mir-21 was higher in the group of reduced PTEN expression (with an M of 6.800) than in the group of high PTEN expression (with an M of 4. 850, P =0. 013). The up-ragulated expression of mir-21 was positively correlated with the TNM clinical stage, lymph node positivity, and proliferation index (P = 0. 021, 0. 010, and 0. 030 respectively). Conclusion mir-21 plays an important role in the development and progression of breast cancer. PTEN is possibly one of the targets of mir-21.
Keywords:Breast neoplasms  MicroRNA  MicroRNA-21  PTEN phosphohydrolase
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号