热疗对人类结肠癌细胞株细胞黏附因子表达的影响

目的 探讨热疗对人类大肠癌细胞株细胞黏附因子表达的影响。方法 采取体外实验方法，在实验室条件下模拟临床热疗条件对HT29细胞株加热43℃，60min，借助PCR及免疫组化方法，分析热疗后不同时间内上皮黏附因子(E-cad)及α-，β-，γ-链蛋白(cat)表达的改变。结果 热疗后24hE-cad表达显著增强，动态分析显示，与未接受热疗的细胞比较，E-cad在热疗后24～72h内呈现逐渐增强趋势；γ-cat的表达在热疗后8～72h内也呈现增强趋势；经过43℃，60min的热疗，β-cat的表达在4～72h内呈现逐渐减弱趋势；α-cat的表达在热疗后未出现显著的改变。结论 43℃，60min的热疗可以上调人类大肠癌细胞株E-cad、γ-cat的表达；下调β-cat的表达；而对α-cat的表达未产生影响。

Change in E-cadherin, alpha-, beta- and gamma-catenin expression after hyperthermia of a human colon carcinoma cell line in vitro

OBJECTIVE: To investigate changes in E-cadherin, alpha-, beta-, and gamma-catenin expression after hyperthermia of a human colon cancer cell line in vitro. METHODS: E-cadherin and alpha-, beta-, and gamma-catenin expression on a human colon carcinoma cell line HT29 at 7 successive times after hyperthermia at 43 degrees C for 60 min were investigated in vitro by RT-PCR and immunochemistry. RESULTS: In vitro studies revealed that E-cadherin expression had increased significantly on HT29 cells at 24 hours after hyperthermia at 43 degrees C for 60 min. In a time-course analysis, the expression of E-cadherin had major increase at 24 to 72 hours after hyperthermia compared with an unheated control cell. gamma-catenin expression had increased significantly at 8 to 72 hours. After hyperthermia at 43 degrees C for 60 min, beta-catenin expression had decreased gradually at 4 hours to 72 hours. alpha-catenin expression kept unchanged after hyperthermia. CONCLUSION: Hyperthermia at 43C for 60 min upregulated E-cadherin and gamma-catenin expressions but downregulated beta-catenin expression on colon carcinoma cells in vitro. alpha-catenin expression was not regulated by hyperthermia.