含β射线的软性血管外模型对移植血管内膜增生及平滑肌增殖的抑 …

目的 观察含β射线＾３２磷（＾３２Ｐ）的软性血管外模型（ＳＥＭ）对移植血管内膜增生（ＩＨ）及平滑肌细胞（ＳＭＣ）增殖的抑制作用。方法 作者在２４吸家兔股静脉－股动脉移植模型上,应用内皮素Ｉ受体基因（ＥＴＡｃＤＮＡ）原位分子杂交及计算机图像分析主动脉在不同条件下（分对照组、阿斯匹林（ＡＳＰ）组和＾３２Ｐ组）,术后（１,２,４,１２周）不同时间内定量观察ＥＴＡｃＤＮＡ表达情况及内膜相对厚度。结果（１）

Soft extravascular model (SEM) with beta-radioactive ray inhibits smooth muscle cell proliferation and intimal hyperplasia in autogenous vein graft

OBJECTIVE: To investigate the effects of the soft extravascular model (SEM) with 32P on intimal hyperplasia and smooth muscle cell preliferation in autogenous vein grafts. METHODS: We transplanted the femoral veins to the co-lateral femoral arteries in rabbits. The animals were divided into three groups of Aspirin group, 32P group and control group. We observed the expression density of endothelin type A receptor cDNA (ETAcDNA) and intimal relative thickness by using in situ molecular hybridization of ETAcDNA by computerized image analysis technology. RESULTS: SEM with beta-ray obviously inhibited SMC preliferation from 1 W to 12 W postoperation, and notably restrained intimal hyperplasia at early to middle stage of graft. Aspirin could not inhibit SMC proliferation and intimal hyperplasia under the condition of this experiment.