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全反式维甲酸致高白细胞症作用机理的初步研究
作者姓名:Chen H  Sun G  Li J
作者单位:上海第二医科大学上海血液学研究所,中国人民解放军空军总医院,镇江医学院附属医院
基金项目:国家自然科学基金,国家“九五”攻关课题基金,胡应洲奖励基金
摘    要:目的探讨全反式维甲酸(ATRA)在体内促外周血白细胞增高作用的细胞生物学机制。方法用3HTdR掺入,祖细胞培养和脾集落形成单位(CFUS)测定等方法,观察在ATRA作用下BALB/C小鼠造血功能的动态变化。结果ATRA可以增加小鼠外周血细胞计数,第8天白细胞计数上升,第16天为对照的183%。骨髓细胞增殖活性增加,3HTdR掺入增多,掺入高峰第8天,为对照的190%,CFUS增殖,12天为对照200%。造血祖细胞粒巨噬细胞集落(CFUGM)增殖,第12天为对照的215%。结论ATRA在小鼠体内具有致白细胞增高作用,这可能与其在体内促CFUGM和CFUS增殖并定向粒系分化有关,与其作用于体内释放造血活性物质如粒细胞集落刺激因子有关。

关 键 词:全反式维甲酸  白细胞增多  白血病.早幼粒细胞.急性

Cellular biological mechanism of leukocytosis produced by all-trans retinoic acid: a preliminary study
Chen H,Sun G,Li J.Cellular biological mechanism of leukocytosis produced by all-trans retinoic acid: a preliminary study[J].National Medical Journal of China,1998,78(4):284-286.
Authors:Chen H  Sun G  Li J
Institution:Ruijin Hospital, Shanghai.
Abstract:Objective To investigate the cellular biological mechanism of leukocytosis produced by all trans retinoic acid (ATRA). Methods BALB/C mice were fed by ATRA. The effects of ATRA on BALB/C mice hematopoiesis was studied by 3H TdR, semi solid progenitor culture, and CFU S number. Results ATRA increased the peripheral blood cells count in vivo. The effect on WBC count was obvious. The peak of the elevation (183% of the control) occurred at the 16th day. Correspondingly, 3H TdR was increased and CFU S was enhanced in BM, and the peak of the elevation (190% and 200% of the control, respectively) occurred at the 8th day. The progenitor cell CFU GM was proliferated and the maximun elevation (215% of control) was observed at the 12th day. Conclusion ATRA could produce leukocytosis in normal BALB/C mice. The effect was possibly related to the proliferation of CFU GM and CFU S and to ATRA induced CSA such as G CSF in vivo.
Keywords:Tretinoin    Leukocytosis    Leukemia  promyelocytic  acute  
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