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粘附分子P选择素单克隆抗体抑制胃癌转移的实验研究
作者姓名:Chen J  Zhang Y  Chu Y
作者单位:上海市第六人民医院,上海市肿瘤研究所,上海第二医科大学附属瑞金医院
摘    要:目的研究P选择素单克隆抗体对SCID小鼠胃癌的抗转移作用。方法用人胃癌组织块SGC7901原位移植SCID小鼠制作转移模型。术后第3天开始,动物分别静脉注射P选择素单克隆抗体(单抗组,9只鼠),每次100μg,每周2次,共3周;或PBS液体(PBS组,11只鼠),每次100μl,每周2次,共3周。术后第6周末,所有动物处死,取肿大淋巴结和可疑转移脏器作病理学检查。结果PBS组肿瘤转移(淋巴结、肝、肺等脏器)率为909%(10/11),单抗组转移率为222%(2/9)。逆转录聚合酶链反应(RTPCR)测定发现,SCID小鼠有癌转移者其胃癌组织中P选择素mRNA基因表达较不伴癌转移者明显增强,正常对照鼠胃组织P选择素mRNA表达水平较低。结论P选择素及其基因mRNA表达与肿瘤转移相关,P选择素单克隆抗体具有抗肿瘤转移作用。

关 键 词:膜糖蛋白类  肿瘤转移  胃肿瘤

Inhibition of human stomach cancer metastasis in vivo by anti-P-selectin monoclonal antibody
Chen J,Zhang Y,Chu Y.Inhibition of human stomach cancer metastasis in vivo by anti-P-selectin monoclonal antibody[J].National Medical Journal of China,1998,78(6):437-439.
Authors:Chen J  Zhang Y  Chu Y
Institution:Shanghai Sixth Hospital.
Abstract:OBJECTIVE: To study the role of cell adhesion molecule P-selectin monoclonal antibody (Mab) in tumor metastasis of an orthotopic implantation metastatic model. METHODS: SCID mice were implanted orthotopically using SGC-7901 human stomach carcinoma intact tissue. Three days later, animals were given i.v. injections of PBS or P-selectin Mab (100 micrograms/injection) twice weekly for 3 weeks. 42 days after operation, all animals were sacrificed. Tissues from stomach and other organs were obtained for histopathological evaluation. RESULTS: Ten of the animals (n = 11) treated with PBS developed metastatic tumors in the regional lymph nodes, liver, and lung. In contrast, 2 of the animals (n = 9) treated with P-selectin Mab developed metastatic tumors in the organs examined. The level of P selectin mRNA expression determined by RT-PCR in gastric carcinoma tissue of SCID mice with tumor metastas is was higher than that without metastasis. CONCLUSIONS: The expression of P-selectin adhesion moleculeis associated with tumor metastasis, and the metastasis can be arrested by the Mab.
Keywords:Membrane glycoproteins    Neoplasm metastasis    Stomach neoplasms
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