西咪替丁对人胃癌细胞SGC-7901生物学行为的影响

目的观察西咪替丁对人胃癌SGC-7901细胞的生长、凋亡、黏附及侵袭行为的影响。方法用四甲基偶氮唑盐（MTr）法检测不同浓度西咪替丁对胃癌SGC-7901细胞生长的影响；流式细胞术检测细胞周期和凋亡；用吖啶橙（AO）染色后荧光显微镜观察药物作用后细胞的形态变化；透射电镜观察用药后细胞超微结构的改变；以matrigel为对象，检测西咪替丁对胃癌细胞与细胞外基质黏附作用的影响；利用millicell小室检测西咪替丁对胃癌细胞侵袭人工基底膜能力的影响。结果在0．5～5mmol／L浓度内，西咪替丁对SGC-7901的生长具有显著的抑制作用，并呈时间和剂量依懒性；0．5～10mmol／L西咪替丁作用后，可观察到典型细胞凋亡形态学改变；流式细胞仪检测可见凋亡峰，G0／G1期细胞明显增多；0．0625～0．25mmol／L西咪替丁可抑制胃癌细胞和细胞外基质的黏附及对重组基底膜的侵袭。结论西咪替丁可改变细胞周期分布，诱导SGC-7901细胞凋亡，从而抑制细胞增殖。在无毒剂量下，可抑制SGC-7901细胞对细胞外基质的黏附与侵袭。

Effects of cimetidine on the biological behaviors of human gastric cancer cells

OBJECTIVE: To investigate the effects of cimetidine on the proliferation, apoptosis, adhesion, and invasion of human gastric cancer cells. METHODS: Human gastric cancer cells of the line SGC-790 were cultured. Cimetidine of the concentrations of different concentrations was added into the culture fluid. The survival rate of the cells was calculated. Flow cytometry was used to examine the distribution of cell cycle and apoptosis of the cells. Fluorescence staining was used to observe the morphology of the apoptotic cells. The adhesion of the cells was measured by MTT method and their invasion ability was tested with Millicell chambers. RESULTS: The proliferation of the AGC-7901 cells was inhibited by cimetidine of the concentration of 0.5 to 5 mmol/L time and dose-dependently. Cimetidine of the concentrations of 0.5 - 10 mmol/L retarded the SGC-7901 cells at the stage G(0)/G(1) and increased the number of apoptotic cells dose-dependently (P < 0.05). Cimetidine of the non-toxic concentrations 250, 125, and 62.5 micrommol/L decreased the adhesion and invasion of the SGC-7901 cells to the extracellular matrix. CONCLUSION: Capable of inducing apoptosis and cell cycle arrest, cimetidine is helpful in treatment of cancers of gastric cancer.