辅助性T细胞极化群体在慢性乙型肝炎病毒感染中的作用

目的 了解慢性乙型肝炎病毒（ＨＢＶ）感染者外周血中辅助性Ｔ细胞（Ｔｈ）内干扰素－γ（ＩＦＮ－γ）、白细胞介素－４（ＩＬ－４）、和转化生长因子－β（ＴＧＦ－β）的表达情况,以测定Ｔｈ１／Ｔｈ２／Ｔｈ３细胞的百分率、探明Ｔｈ细胞各极化群体在慢性ＨＢＶ感染中的作用。方法 将３６例 性ＨＢＶ感染者分２例,慢性ＨＢＶ感染组和中度慢性乙型肝炎组,每组１８例患者。正常对照组１２名。常规分离外周血单个核然佛波二脂

Polarized populations of T helper cells in patients with chronic hepatitis B virus infection

OBJECTIVES: To investigate the composition of Th1/Th2/Th3 cells in chronic HBV-infected individuals by determining the expression of IL-4/IFN-gamma/TGF-beta in single CD4+ T cell isolated from the peripheral blood mononuclear cells (PBMCs), and analyze the role of Th cells polarized population in chronic HBV-infection. METHODS: PBMCs from chronic HBV infected individuals were separated routinely, stimulated by PMA/Ionomycin/Monensin, and the production of IL-4/IFN-gamma/TGF-beta by CD4+ T cells in PBMCs was determined by FACS analysis. RESULTS: The percentage of IFN-gamma-producing T cells, IL-4-producing T cells and TGF-beta producing T cells ranged from 2.3%-18.6%, 1.1%-8.7% and 0.7%-7.1%, respectively, in CD4+ cells from non-infected individuals. The majority of CD4+ T cells from PBMCs in chronic HBV-infected individuals were Th0 cells. The proportion of Th1 cells increased significantly with the hepatic inflammation activity, and the proportion in active period of chronic hepatitis B was higher than that in nonactive period (P < 0.05). The percentage of Th2 cells in CD4+ T cells from HBV-infected individuals did not differ significantly (P > 0.05), but it was higher than controls (P < 0.05). The percentage of Th3 cells in CD4+ T cells from AsC group was higher than that in CHB group and control (P < 0.05). CONCLUSIONS: Th1 phenotype cytokine are correlated with hepatic inflammation activity of chronic hepatitis B. Th2 cells may be associated with the persistence of HBV infection. Th3 cells cooperating with Th2 cells exert negative immunoregulating action and may be associated with the immune tolerance state of chronic HBV infection.