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促性腺激素释放素及其受体在人肝癌组织中的分布及意义
作者姓名:Zhang J  Huang G  Huang W
作者单位:第四军医大学基础部组织胚胎学教研室
基金项目:“九五”军队医药卫生科研基金
摘    要:目的观察促性腺激素释放素(GnRH)及其受体在不同分化程度肝癌组织中的定位与分布,为研究GnRH与肝癌细胞增殖调控的关系提供依据。方法用常规组织学HE染色和免疫组织化学链霉亲合素生物素化过氧化物酶复合物(SABC)技术检测了33例人肝细胞性肝癌及其癌旁肝组织中GnRH及其受体的表达和分布情况。结果GnRH与GnRH受体在肝癌细胞和癌旁肝细胞的定位及分布范围基本一致,阳性物质主要位于癌细胞的胞膜和胞浆,核周区染色增强,胞核未见阳性产物。分化Ⅰ、Ⅱ级肝癌组织GnRH的阳性率为94%(16/17),高于II级肝癌[50%(5/10,P<005)和Ⅳ级肝癌[17%(1/6)P<0001]。Ⅰ~Ⅱ级肝癌组织GnRH受体阳性率为88%(15/17),高于Ⅳ级肝癌[17%(1/6)P<001]。此外,尽管各分化级别肝癌组织中GnRH及其受体的阳性率均低于其相应癌旁肝硬化组织,但差异无显著意义(P>005)。结论肝癌组织中GnRH及其受体的表达与其分化程度有关。

关 键 词:癌.肝细胞  受体.促性腺激素释放素  免疫组织化学

Gonadotropin releasing hormone and its receptor in the tissue of human hepatocellular carcinoma
Zhang J,Huang G,Huang W.Gonadotropin releasing hormone and its receptor in the tissue of human hepatocellular carcinoma[J].National Medical Journal of China,1998,78(5):343-346.
Authors:Zhang J  Huang G  Huang W
Institution:Department of Histology and Embryology, Fourth Military Medical University, Xian.
Abstract:OBJECTIVE: To provide the basis for studying the relationship between gonadotropin releasing hormone(GnRH) and the regulation of hepatocarcinoma cell proliferation and differentiation, we observed the localization and distribution of GnRH and its receptor(GnRH-R) in various differentiated hepatocarcinoma tissues. METHODS: GnRH and its receptor were detected in 33 hepatocellular carcinoma(HCC) by immunohistochemical SABC technique. RESULTS: GnRH and its receptor had the same distributive pattern in hepatocarcinoma cells. The positive signal was found mainly on the surface and in the cytoplasm of hepatoma cells, with enhanced staining in the cytoplasm surrounding the nucleus; no positive signal was revealed in the nucleus. The GnRH positive rate (94%; 16/17) of edmondson grade I-II HCC was higher than that of grade III HCC (50%; 5/10) and grade IV HCC(17%; 1/6), (P < 0.05 and P < 0.001 respectively). In grades I-II HCC GnRH-R positive rate was 88% (15/17), which was higher than 17% (1/6) in grade IV HCC (P < 0.01). Although GnRH and GnRH-R positive rates in every grade HCC were lower than those in their related peri-cancerous tissues, the differences were not significant (P > 0.05). CONCLUSION: The expression of GnRH and GnRH-R in the tissues of hepatocarcinoma is related to their degree of differentiation.
Keywords:Hepatocellular  carcinoma    Receptors  Gonadoliberin    Immunohistochemistry  
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