| Nogo-A mRNA在大鼠神经组织中的表达和定位 |
| |
| 作者姓名： | Ye J Wang Z Zhu P |
| |
| 作者单位： | 400042,重庆,第三军医大学大坪医院野战外科研究所 |
| |
| 基金项目： | “九七三”国家重点基础研究发展规划基金资助项目(G19990 5 42 0 4),军队十五医学科研基金资助项目 (0 1MA175 ) |
| |
| 摘 要： | 目的：观察Nogo-A mRNA在脑,脊髓,视神经,坐骨神经的表达和细胞定位。方法：采用原位杂交技术观察8只正常Wistar大鼠脑,脊髓,视神经,坐骨神经中Nogo-A mRNA的表达和定位,结果：8只大鼠脑,脊髓,视神经切片中Nogo-A mRNA均表面阳,是性信号位于胞浆,胞核不着色,8只大鼠坐骨神经切片中均表达阴性。结论：在大鼠中枢神经如脑,脊髓,视神经中Nogo-A mRNA广泛分布,而在周围神经坐骨神经则无Nogo-A mRNA表达,提示Nogo-A mRNA阳性表达及分布与中枢神经系统再生功能低下密切相关。
|
| 关 键 词： | 周围神经损伤 神经生长因子 基因表达 Nogo－A mRNA |
| 修稿时间： | 2001年10月24 |
Expression and localization of Nogo-A mRNA in rat nerve tissue |
| |
| Ye J,Wang Z,Zhu P.Expression and localization of Nogo-A mRNA in rat nerve tissue[J].National Medical Journal of China,2002,82(7):498-500. |
| |
| Authors: | Ye Jian Wang Zhengguo Zhu Peifang |
| |
| Institution: | Research Institute of Field Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. |
| |
| Abstract: | Objective To investigate the expression and localization of Nogo A mRNA in brain, spinal cord, optic nerve, and sciatic nerve. Methods Frozen sections of brain, spinal cord, optic nerve, and sciatic nerve of 8 rats were made. In situ hybridization was conducted and the sections were observed by light microscopy. Results The expression of Nogo A mRNA was positive in brain, spinal cord, and optic nerve, and negative in sciatic nerve. Conclusion The expression of Nogo A mRNA is negative in brain, spinal cord, and optic nerve, which may be related to the poor axon regeneration in central nervous system. |
| |
| Keywords: | Central Nervous system Peripheral nervous system Nerve growth factor Gene expression |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
|
