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肝素对大鼠肾小球系膜细胞的抑制作用
引用本文:张明,郭慕依.肝素对大鼠肾小球系膜细胞的抑制作用[J].中华医学杂志,1995,75(5):273-275.
作者姓名:张明  郭慕依
作者单位:上海医科大学病理生理教研室
摘    要:通过观察肝素对体外培养的大鼠肾不球系膜细胞生长、超微结构、细胞外基质合成及癌基因表达的影响,探讨肝素减轻以MsC增生为特征的肾小球病变的可能怀。分离培养的MsC经鉴定后,分为肝素组和对照组进行观察,结果发现肝素可明显抑制MsC生长,此WTET具有剂量依赖性,电镜发现肝素使MsC微丝减少,内质网和高尔基体扩张,次组溶酶体增多,髓样小体形成。

关 键 词:肾小球肾炎  肝素  膜细胞  

Inhibitory effect of heparin on glomerular mesangialcell
Zhang Ming, Guo Muyi, Jin Huiming et al..Inhibitory effect of heparin on glomerular mesangialcell[J].National Medical Journal of China,1995,75(5):273-275.
Authors:Zhang Ming  Guo Muyi  Jin Huiming
Abstract:he pure mesangial cells (MsC) were isolated fromthe cuhure of rat glomeruli. After dentification, theywere divided into heparintreatment group and controlgroup. It was found that heparin profoundly inhibitedthe growth of MsC with 75. 9% inhibitory rate bymeans of direct counting MsC. MTT method provedthat the inhibitory effect of heparin on MsC was dose-dependent. Electronmicroscopic observation revealed di-lated endoplasmic reticulum and Golgi vesicles, moresecondary lysosomes, prominent myelin figures and decreased lmicrofilaments in the cytoplasm of the MsC ex-posed to heparin. Immunocytochemical studies showedthat the amounts of FN and LM in the cytoplasm ofMsC decreased after heparin treatment. Immunocytochemical investigation also confirmed that heparincould inhibite the expression of nuclear oncogene cfosand cjun in the MsC stimulated by PMA. The signifi-cance and possible mechanism of inhibitory effect of hep-arin on MsC were discussed.
Keywords:Glomerulonephrrtis  membranopro-lif-erative Heparin fibronectin laminin Mensangialcell  
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