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Par-4基因沉默对人骨髓间充质干细胞中Smac基因表达和半胱氨酸蛋白水解酶3酶活性的影响及其抗凋亡作用
作者姓名:Lu C  Chen JQ  Zhou GP  Wu SH  Guan YF  Yuan CS  Huang SM  Guo XR  Chen RH
作者单位:1. 南京医科大学第一附属医院儿科,210029
2. 南京医科大学儿科研究所
基金项目:国家自然科学基金,江苏省卫生厅医学科技发展重大基金 
摘    要:目的 研究Par-4基因沉默对人骨髓间充质干细胞(hBMSC)中Smac基因表达和半胱氨酸蛋白水解酶(Caspase)3酶活性的影响及其抗凋亡作用.方法 原代培养hBMSC.谷氨酸诱导hBMSC凋亡.设计和合成两对针对Par-4基因mRNA的小RNA干扰(siRNA)序列(Par-4-SiRNA-1、2),构建真核细胞表达载体,用脂质体介导转染hBMSC,利用G418筛选稳定表达细胞株.实时荧光定量PCR法检测Par-4 mRNA表达水平.流式细胞术测定细胞凋亡百分率.Western印迹测定Smac蛋白表达量.比色法测定Caspase-3酶的活性.结果 设计的Par-4-SiRNA-1、2可显著诱导hBMSC中Par-4基因沉默,Par-4 mRNA水平分别被降低88%、67%,谷氨酸诱导hBMSC凋亡,凋亡百分率为(58.9±8.9)%.Par-4-SiRNA-1可显著拮抗谷氨酸的诱导凋亡作用,凋亡百分率降为(37.2±6.3)%(F=58.26,P<0.01).Par-4-SiRNA-1对谷氨酸诱导的hBMSC中Smac蛋白表达上调(P<0.01)和Caspase-3酶活性上调(P<0.01)有显著的抑制作用(P<0.01).结论 Par-4基因沉默能拮抗谷氰酸对hBMSC凋亡的诱导作用.其机制可能与Smac基因表达和Caspase-3酶活性被抑制有关.

关 键 词:干细胞  脱噬作用  基因  Par-4

Effects of Par-4 gene silencing induced by siRNA on the expression of Smac gene, activity of caspase-3, and apoptosis of human bone marrow mesenchymal stem cells
Lu C,Chen JQ,Zhou GP,Wu SH,Guan YF,Yuan CS,Huang SM,Guo XR,Chen RH.Effects of Par-4 gene silencing induced by siRNA on the expression of Smac gene, activity of caspase-3, and apoptosis of human bone marrow mesenchymal stem cells[J].National Medical Journal of China,2008,88(6):411-415.
Authors:Lu Chao  Chen Ji-Qing  Zhou Guo-Ping  Wu Sheng-Hua  Guan Ya-Fei  Yuan Chuan-Shun  Huang Song-Ming  Guo Xi-Rong  Chen Rong-Hua
Institution:Department of Pediatrics, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. luchao8009@163.com
Abstract:OBJECTIVE: To investigate the effects of Par-4 gene silencing induced by siRNA on the expression of Smac gene, activity of caspase-3, and apoptosis of human bone marrow mesenchymal stem cells (hBMSCs). METHODS: Bone marrow was obtained from a healthy young man and hBMSCs were isolated and cultured. Two siRNAs (Par-4-siRNA-1 and -2) targeting Par-4 gene were chemically synthesized. Eukaryocytic expression vectors containing these Par-4 siRNA sequences were established and transfected into the hBMSCs. The hBMSCs were divided into 4 groups: non-transfected hBMSCs (normal control group), blank Pae-4 plasmid transfected hBMSCs (Par4 control group), Par4-siRNA-1 transfected hBMSCs, and Par-4-siRNA-2 transfected hBMSCs. The expression of Par-4 mRNA was detected by real-time PCR. Another hBMSCs were inoculated in DMEM and divided into 4 groups: non-transfected normal hBMSCs, glutamate (an apoptosis inducer) + non-transfected hBMSC group, glutamine + Par-4-siRNA-1 hBMSC group, and glutamate + Par4-SiRNS-2 hBMSC group. Flow cytometry was used to detect the apoptotic rate. The relative activity of caspase-3 was determined by colorimetric assay. Western blotting was used to detect the Smac protein expression. RESULTS: The relative mRNA expression levels of Par-4 gene of the Par-4-siRNA-1 hBMSCs, Par-4 SiENA-2 hBMSCs, and Par-4 control hBMSCs were 0.12 +/- 0.03, 0.33 +/- 0.09, and 0.97 +/- 0.02 respectively, decreased by 88%, 67%, and 3% respectively compared with that of the normal control. The percentages of apoptotic cells of the glutamate + Par-4-siRNA-1 hBMSCs was (37.2 +/- 6.3)%, significantly lower than that of the glutamate + non-transfected hBMSC group (58.9 +/- 8. 9)%, F = 58.26, P < 0.01). The Smac protein expression level of the glutamate + non-transfected hBMSC group was significantly higher than that of the normal control group (P < 0.01); however, the Smac protein expression level of the Par-4-siRNA-1 hBMSC group was significantly lower than that of the glutamate + non-transfected hBMSC group (P < 0.01). The caspase-3 activity of the glutamate + Par-4-siRNA-1 hBMSC group was significantly lower than that of the glutamate + non-transfected BMSC group (P < 0.01). CONCLUSION: Par-4-siRNA-1 inhibits markedly the apoptosis of the hBMSCs induced by glutamate. Par-4 gene silencing induced by siRNA inhibits the apoptosis of hBMSCs. The mechanism of the inhibition may be closely related to suppression of the up-regulation of Smac gene expression and caspase-3 activity.
Keywords:RNA
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