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bcl-2基因转染小鼠造血干细胞重建造血的生物学特性
作者姓名:Zhao J  Chu J  Ding S
作者单位:中国医学科学院中国协和医科大学血液学研究所
摘    要:目的 观察bcl-2基因转染小鼠造血干细胞重建造血的小鼠对放化疗的抵抗作用。方法 (1)含bcl-2cDNA的逆转录病毒载体质粒,经脂质体介导转染PA317包装细胞,通过G418筛选和克隆,获得高病毒滴度的细胞株(D3);(2)用D3细胞株的病毒上清转染小鼠胚胎肝造血干细胞;(3)经致死剂量照射小全注bcl-2转 造血干细胞重建造血;(5)采用脾集落形成和再增殖能力观察bcl-2转因干细胞重建造血

关 键 词:白血病  造血干细胞  抗药性  重建造血  转基因动物

Biological properties of reconstructed mouse hematopoiesis with hematopoietic stem cells transfected by bcl-2 gene
Zhao J,Chu J,Ding S.Biological properties of reconstructed mouse hematopoiesis with hematopoietic stem cells transfected by bcl-2 gene[J].National Medical Journal of China,1999,0(1):50-53.
Authors:Zhao J  Chu J  Ding S
Institution:Institute of Hematology, State Key Lab. of Experimental Hematology, CAMS, PUMC, Tianjin 300020.
Abstract:Objective To observe resistance to radiochemical agents in the mice reconstructed hematopoiesis with hematopoietic stem cells transfected by bcl 2 gene.Methods The plasmid containing bcl 2 cDNA retrovirus vector was transfected to PA317 cells with Lipofecaine TM kite. A PA317 cell clone(D3) with high virus titer was obtained through G418 selection. The virus supernatant of D3 clone cells was used to transfect mouse embryo liver hematopoietic stem cells. Hematopoiesis in the mice treated with lethal dose irradiation was reconstructed by hematopoietic stem cells transfected with bcl 2 gene. The resistance to radiochemical agents in the mice with reconstructed hematopoiesis was evaluated according to the number of WBC of periphereal blood and mononuclear cells of bone marrow, CFU S and CFU S regeneration. Results The expression of bcl 2 protein was increased in the hematopoietic cells transfected by bcl 2 gene.The mice reconstructed hematopoiesis by stem cells transfected bcl 2 gene can tolerate high dose combined with chemotherapy and radiation of middle dose. The mice reconstructed hematopoiesis with stem cells transfected bcl 2 gene survived over one year, no tumors were found.Conclusion The mice with reconstructed hematopoiesis by stem cells carried bcl 2 gene possess the ability of resistance to radiochemical agents, even only part of hematopoietic stem cells were transfected with bcl 2 gene.
Keywords:Mice  transgenic    Hematopoietic stem cell    Drug resistance  
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