转化生长因子大黄酸对肾小球系膜细胞葡萄糖转运蛋白功能的影响

目的 对人肾小球系膜细胞葡萄糖转运蛋白－１（ＧＬＵＴ１）进行鉴定。研究ＧＬＵＴ１的作用特点，ＴＧＦ－β１对其影响以及大黄酸的干预作用。方法 分别用ＲＴ－ＰＣＲ，免疫荧光染色，流式细胞仪和［＾３Ｈ］－２脱氧葡萄糖摄入率，对系膜细胞ＧＬＵＴ１ｍＲＮＡ表达，蛋白质分布和功能进行鉴定。观察不同浓度ＴＧＦ－β１在加或不加大黄酸的情况下对系膜细胞葡萄糖摄入以及ＧＬＵＴ１ｍＲＮＡ表达的影响。结果 人类肾小球系膜

Modulatory effect of transforming growth factor-beta and Rhein on glucose transporter-1 in human glomerular mesangial cells]

OBJECTIVES: To identify the glucose transporter-1 (GLUT1) in human glomerular mesangial cell and to evaluate the modulatory role of TGF-beta 1 and Rhein on the function of GLUT1 in mesangial cells. METHODS: GLUT1 in human glomerular mesangial cells was identified by RT-PCR for its mRNA expression. Cell surface protein of GLUT1 had been demonstrated by immunofluorescence staining and flow cytometry analysis using specific antibody. The uptake rate of glucose by mesangial cells was detected using the 3H]-2-DG. Glucose uptake specificity was confirmed by the co-incubation with unlabeled 2-DG or GLUT1 inhibitor Phlorhizin. RESULTS: Human glomerular mesangial cells expressed functional GLUT1. TGF-beta 1 stimulated 3H]-2-DG uptake and GLUT mRNA expression in mesangial cells. The increase in 3H]-2-DG uptake and GLUT1 mRNA expression by TGF-beta 1 in mesangial cells were markedly abolished by the addition of Rhein in a dose dependent manner, while Rhein showed no effect on the glucose uptake in mesangial cells cultured in normal glucose medium. CONCLUSIONS: Functional GLUT1 does present in human mesangial cells. TGF-beta 1 stimulates the glucose uptake by enhancing the GLUT1 mRNA expression in mesangial cells. This effect could be antagonized by Rhein. These results suggest that Rhein might be a hopeful drug for patients with diabetic nephropathy.