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缺氧缺血大鼠脑细胞凋亡的研究
作者姓名:Jiang L  Tang Y  Huang X
作者单位:南京铁道医学院附属医院儿科,南京铁道医学院科学研究所分子生物学研究室
摘    要:目的探讨脑细胞凋亡在缺氧缺血脑病(HIE)发病机理中的作用。方法用HE染色、电镜、原位末端标记(Tunel)手段,分别对缺氧3小时后缺血1、4、18、24、40及72小时和7天组大鼠的脑组织中凋亡细胞的形态、出现时间及密度进行观察和比较。结果光、电镜下显示实验侧脑皮质、海马及丘脑神经元皱缩、染色质凝集并出现凋亡小体;Tunel方法证实有裂解DNA存在;缺氧缺血后18小时皮质凋亡最明显。结论在HIE大鼠,脑细胞凋亡出现不但早于坏死,而且与坏死存在着诱导剂量相关性

关 键 词:脑缺氧  脑缺血  脱噬作用  大鼠

Brain cell apoptosis after cerebral hypoxia-ischemia in neonatal rat
Jiang L,Tang Y,Huang X.Brain cell apoptosis after cerebral hypoxia-ischemia in neonatal rat[J].National Medical Journal of China,1998,78(8):567-569.
Authors:Jiang L  Tang Y  Huang X
Institution:Department of Pediatrics, Nanjing Railway Medical College.
Abstract:OBJECTIVE: To investigate action of apoptosis in mechanisms of neonatal hypoxia-ischemia (HIE). METHODS: Using HE staining, electronmicroscope, and terminal deoxynuleotidyl transferase-mediated dUTP nick end labeling (TUNEL) technique, we observed the histological features, time course and density of apoptotic cells and compared the ipsilateral hemisphere after permanent ischemia 1, 4, 18, 24, 40, 72 h, 7 days after 3 h hypoxia with that of sham-group. RESULTS: Neurons in the cortex, hippocampus, thalamus exhibited cells shrink, chromatin condensation, apoptosis bodys under microscope, as demonstrated by in situ labeling of DNA breaks. The peak for apoptosis was shown at 18 h in the cortex. CONCLUSION: In the HIE, apoptosis appears earlier than necrosis, and both of the different cell death forms may exist induced dose relativity.
Keywords:Cerebral anoxia    Cerebral ischemia    Apoptosis    Rats  
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