Rb基因对血管平滑肌细胞生长抑制作用的研究

目的探讨腺病毒载体介导的Ｒｂ基因对血管平滑肌细胞的作用，及其应用于动脉粥样硬化及动脉再狭窄基因治疗的可能性。方法构建Ｒｂ基因的重组腺病毒载体，体外转染兔血管平滑肌细胞。应用生化染色、免疫组化及聚合酶链反应检测基因的转染效率、基因表达情况。应用细胞计数、同位素掺入、流式细胞仪检测手段，观察Ｒｂ基因对平滑肌细胞生长的作用。结果腺病毒载体能快速有效地将外源性基因导入平滑肌细胞并表达。Ｒｂ基因导入可抑制平滑肌细胞生长，减少细胞ＤＮＡ合成，减少合成后期和分裂期细胞量，增加静止期和合成前期细胞。结论腺病毒载体介导Ｒｂ基因导入有可能作为动脉粥样硬化及动脉再狭窄的基因治疗手段。

The inhibition effect of foreign retinoblastoma gene mediated by recombinant adenovirus vector on the growth of smooth muscle cells

Objective\ To study the effect of foreign retinoblastoma(Rb)gene mediated by recombina adenovirus vector on vascular smooth muscle cell(SMC)and the possibility by using Rb gene for gene therapy of atherosclerosis and artery restenosis. Methods\ Rb gene recombinant adenovirusvector was con structed to transfect into rabbits' aortic SMC in vitro.The efficiency of transfection and expression was detected by biochemical staining,immunocytochemical analysis and polymerase chain reaction tech nique.The role of Rb gene for SMC proliferation,DNA synthesis and cell cycle were observed by cellcounting, 3H incorporation and cytometer respectively.Results\ Adenovirus vector transfered the Rb gene into SMC effectively.The expression of Rb gene restricted the proliferation of SMC,decreased the DNA synthesis and plaaied arole in the cell cycle Conclusion\ Rb gene mediated by adenovirus can be used to treat atherosclerosis.