首页 | 本学科首页   官方微博 | 高级检索  
检索        

结直肠癌组织中K-ras基因的检测及其意义
引用本文:徐向明,张国良,李敏伟,王丹阳,林建江.结直肠癌组织中K-ras基因的检测及其意义[J].中华医学杂志,2010,90(18).
作者姓名:徐向明  张国良  李敏伟  王丹阳  林建江
作者单位:浙江大学医学院附属第一医院肛肠外科浙江省结直肠疾病诊治中心,杭州,310003
摘    要:目的 观察结直肠癌组织中K-ras基因的表达情况,并分析其与临床病理学的相互关系,为临床筛选出能从生物靶向治疗中获益的人群.方法 对208例结直肠癌组织标本,使用聚合酶链式反应(PCR)技术扩增目的基因(包含12和13密码子),并对扩增产物进行Sanger法测序,进行K-ras基因的检测,结合相关的临床病理学资料,分析K-ras基因的表达状况同临床病理学的关系.结果 208例结直肠癌组织中K-ras基因12或13密码子突变为91例,突变率为43.8%,突变率在男女性别之间、肿瘤位置、组织分化类型和Duke's分期间差异无统计学意义.结论 对结直肠癌组织K-ras基因的表达状况进行检测有助于临床筛选出针对抗EGFR靶向治疗药物有效的结直肠癌患者.

关 键 词:结直肠肿瘤  基因  ras  靶向治疗

Significance of K-ras detection in colorectal cancer
XU Xiang-ming,ZHANG Guo-liang,LI Min-wei,WANG Dan-yang,LIN Jian-jiang.Significance of K-ras detection in colorectal cancer[J].National Medical Journal of China,2010,90(18).
Authors:XU Xiang-ming  ZHANG Guo-liang  LI Min-wei  WANG Dan-yang  LIN Jian-jiang
Abstract:Objective To determine the mutation status of K-ras gene in colorectal cancer and analyze the associations between its mutation status and clinicopathological characteristics in colorectal cancer so as to select the patients likely to benefit from a targeted therapy. Methods A total of 208 colorectal cancer tissue samples were collected from September 2008 to February 2009. DNA was extracted with a genomic DNA miniprep kit. Then PGR was performed with the designed primers and the product directly sequenced by the Sanger method. Then the associations between K-ras mutation status and clinicopathological characteristics in colorectal cancer were analyzed. Results Of 208 cases, 91 cases of Kras gene mutation were detected. The 12 or 13 codon had a mutation rate of 43.8%. There were no significant differences in gender, tumor location, histopathological grading and Duke's stage between the wild and mutated groups. Conclusion Detection of K-ras gene status in colorectal cancer will help to select the patients likely to benefit from the monoclonal antibody therapy of targeting epidermal growth factor receptor.
Keywords:Colorectal neoplasms  Gene  ras  Targeted therapy
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号