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长程吸入一氧化氮对大鼠缺氧性肺动脉高压的影响
作者姓名:Xiong C  Cheng X  He J
作者单位:中国医学科学院中国协和医科大学阜外心血管病医院心脏肺循环研究室
摘    要:目的探讨长程吸入一氧化氮(NO)对慢性缺氧性肺动脉高压的阻抑效应及其机制。方法将32只雄性Wistar大鼠分为(1)正常对照组;(2)缺氧组;每天缺氧12小时,共3周;(3)吸入NO组:缺氧同时每天吸入20ppmNO12小时,共3周。观察肺动脉压力、右心室肥厚、血浆内皮素-1(ET-1)和环磷酸鸟苷(cGMP)变化以及肺组织病理学改变。结果(1)缺氧组大鼠肺动脉压力明显升高(4.3±0.9kPa),右心室肥厚显著(P均<0.001),吸入NO组肺动脉压力降低(3.0±0.4kPa),右心室肥厚减轻;(2)缺氧组腺泡内肌型肺动脉增多,无肌肺动脉明显减少,与对照组相比,差异显著(P<0.01),吸入NO组明显改善三型血管比例失调。(3)缺氧组血浆ET-1含量明显升高,(197±15pg/ml),cGMP水平显著降低。吸入NO组ET-1含量降低(163±14pg/ml);cGMP含量升高。结论长程吸入20ppmNO能阻抑慢性缺氧性肺动脉压力升高,且无明显毒副作用。

关 键 词:缺氧  一氧化氮  缩血管肽类

Effect of prolonged inhalation of nitric oxide on chronically hypoxic rats
Xiong C,Cheng X,He J.Effect of prolonged inhalation of nitric oxide on chronically hypoxic rats[J].National Medical Journal of China,1998,78(1):19-22.
Authors:Xiong C  Cheng X  He J
Institution:Department of Cardiopulmonary, Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing.
Abstract:Objective To explore the effect of prolonged nitric oxide (NO) inhalation on chronically hypoxic rats. Methods Male Wistar rats were randomly divided into 3 groups:1) room air control (C); 2) hypoxia (H); 3) hypoxia with the inhalation of 20ppm NO(2NH). RV/LV S, mPAP and the histopathology of the lung were examined. Plasma levels of ET 1 and cGMP were detected by radioimmunoassy. Results 1. Hypoxia caused marked elevation in mPAP and RV/LV s (both P <0.001 vs Group C). NO significantly reduced mPAP and RV/LV S; 2. Hypoxia raised the percentage of intra acinar muscular artery (MA) and lowered that of nonmuscular artery (NAM). MA decreased from 35.2% to 16.8% in Group 2NH; NMA increased from 43.6% to 64.8% in Group 2NH, 3. The plasma level of ET 1 increased and that of cGMP droped significantly in Group H(both P <0.001 vs Group C). NO resulted in a marked reduction in the plasma ET 1 and an increase in plasma cGMP; 4. Inhalation of NO had no effect on methemoglobin and lung index in hypoxic rats. Conclusions Prolonged inhalation of 20ppm NO can attenuate hypoxic pulmonary hypertension and inhibit the hypertrophy of the pulmonary vascular smooth muscle and the right ventricle NO, when inhalated at a concentration of 20ppm for a long duration, will cause no toxicity in chronically hypoxic rats.
Keywords:Hypoxia    Nitric oxide    Endothelin  
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