激活诱导细胞死亡在乙型肝炎发病机制中的意义

目的　研究激活诱导细胞死亡 (AICD)在乙型肝炎发病机制中的意义。方法　分离 10名健康献血员的外周血单个核细胞 (PBMC) ,设置加HBVDNA阳性血清组与加健康人血清对照组(HBVDNA阴性血清组 ) ,在体外培养 72h ,采用碘化丙啶染色法经流式细胞仪检测凋亡情况并相互比较 ;分离 14例慢性乙型肝炎、6例慢性重型乙型肝炎患者与 10例健康献血员PBMC ,在植物血凝素刺激下培养 72h ,采用碘化丙啶染色法经流式细胞仪检测凋亡情况并相互比较。结果　加HBVDNA阳性血清组培养PBMC凋亡率高于加健康人血清组培养细胞凋亡率 (39 5 6± 7 0 3) %vs(2 7 5 7± 7 78) % ,P <0 0 5 ]。慢性乙型肝炎组PBMC凋亡率 (30 5 7± 13 4 3) % ]高于正常对照组PBMC凋亡率 (11 4 5± 5 2 7) % ,P <0 0 1],高于慢性重型乙型肝炎组PBMC凋亡率 (13 5 9±6 4 4 ) % ,P <0 0 1]。结论　HBV感染可能具有诱导PBMC凋亡的能力 ,AICD可能是形成HBV慢性感染免疫耐受的一个重要机制。

The significance of activation-induced cell death (AICD) in pathogenesis of hepatitis B

OBJECTIVE: To explore the significance of activation-induced cell death (AICD) in pathogenesis of hepatitis B. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from 10 healthy blood donors. PBMCs from one person were divided into two samples. One sample was incubated with the HBV-DNA positive serum. The other was incubated with the serum of healthy person (HBV-DNA negative serum). After 72 hours incubation, the apoptosis of the PBMCs was assayed by the method of propidium iodide (PI) staining with flow cytometry. PBMCs were isolated from 14 patients with chronic hepatitis B, 6 patients with chronic severe hepatitis B and 10 healthy blood donors. The PBMCs were incubated with PHA-P. After 72 hours incubation, the apoptosis of the PBMCs was assayed by the method of PI staining with flow cytometry. RESULTS: The apoptotic rate of PBMCs incubated with HBV-positive serum was significantly higher than that with the serum of normal persons (39.56 +/- 7.03)% vs (27.57 +/- 7.78)%, P < 0.02]. The apoptotic rate of PBMCs of patients with chronic hepatitis B (30.57 +/- 13.43%) was significantly higher than that of healthy blood donors (11.45 +/- 5.27)%, P < 0.01] and higher than that of the patients with chronic severe hepatitis B (13.59 +/- 6.44)%, P < 0.01]. CONCLUSION: HBV infection induces apoptosis of PBMC. AICD may be one of the important mechanisms of peripheral immune tolerance in HBV chronic infection.