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应用mRNA差异显示技术筛选卵巢癌耐药相关基因
引用本文:李袭,邓庆丽,傅祖植.应用mRNA差异显示技术筛选卵巢癌耐药相关基因[J].中华医学杂志,2000,80(7):541-543.
作者姓名:李袭  邓庆丽  傅祖植
作者单位:[1]中国人民解放军总医院妇产科 [2]中国人民解放军军事医学科学院
摘    要:目的 筛选卵巢癌泰素耐药相关基因。方法 采用间歇诱导法建立了卵巢癌泰素耐药细胞株Skov3/Taxl-25;采用mRNA DD比较Skov3/Taxol-25对泰素耐药增加44倍,同时对顺铂也产生了耐药。比较Skov3和Skov3/Taxol-25的mRNA,获得22个差异条带。对其中5个差异条带进行了DNA序列测定,有3个与已知的基因高度同源,分别为tPA、Kiaa0372和LDLC。2个为未知

关 键 词:卵巢肿瘤  抗药性  mRNA差异显示  而药基因
修稿时间:1999-07-07

The selection of taxol resistance associated genes in ovarian cancer cell line by mRNA DD
G Cheng,F Tian,Y Li.The selection of taxol resistance associated genes in ovarian cancer cell line by mRNA DD[J].National Medical Journal of China,2000,80(7):541-543.
Authors:G Cheng  F Tian  Y Li
Institution:Department of Obstetrics & Gynecology, Chinese PLA General Hospital, Beijing, 100853, China.
Abstract:Objective One of the main reasons of the treatment failure for ovarian cancer is the emergence of drug resistance in which several genes involved. The purpose of the study was to select the genes related to Taxol resistance in ovarian cancer. Methods Resistant ovarian cancer cell line, Skov3/Taxol 25, was established by interval exposure Skov3 to Taxol. mRNA was compared between Skov3 and Skov3/Taxol 25 by mRNA DD. Results Skov3/Taxol 25 showed 44 times increase resisance to Taxol after 12 months of induction. There were 22 new differential bands obtained from Skov3/Taxol 25 as compared with Skov3. 18 bands were special by repeated PCR. In the 5 sub clones, three clones were homologous to the known gene. 105/106 base pair of clone 1 were homologous to tPA (tissue plasminogen activator), 261/268 of clone 3 to Kiaa 0372, and 406/409 of clone 18 to LDLC. The other 2 ESTs were new gene segments, which were banked to Genbank with the number of AF120327 and AF120328. The identification by RT PCR revealed that both tPA and Kiaa0372 were special genes from Skov3/Taxol 25, while LDLC was pseudopositive. Conclusion Several genes were involved in the resistance to Taxol in ovarian cancer. The enhanced expression of tPA indicated the increased ability of metastasis after the acquisition of resistance to taxol.
Keywords:Ovarian neoplasms  Drug resistance  Genes
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