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硫酸氨基葡萄糖对大鼠软骨细胞复制性老化的作用
引用本文:陈晓东,林建华.硫酸氨基葡萄糖对大鼠软骨细胞复制性老化的作用[J].包头医学院学报,2009,25(1):1-4.
作者姓名:陈晓东  林建华
作者单位:1. 深圳平乐骨伤科医院脊柱外科,广东,深圳,518010
2. 福建医科大学附属第一医院
基金项目:福建省科技计划重大项目 
摘    要:

关 键 词:硫酸氨基葡萄糖  大鼠  软骨细胞  老化

The Role of Glucosamin Sulphate in Replicative Senescence of Rat Chondrocyte
CHEN Xiaodong,LIN Jianhua.The Role of Glucosamin Sulphate in Replicative Senescence of Rat Chondrocyte[J].Journal of Baotou Medical College,2009,25(1):1-4.
Authors:CHEN Xiaodong  LIN Jianhua
Institution:CHEN Xiaodong,LIN Jianhua (Department of Spine Surgery,Shenzhen Pingle Hospital of Orthopedics , Traumatology,Shenzhen 518010,China)
Abstract:Objective: To observe the effect of glucosamin sulphate (GS) on replicative senescence of rat chondrocyte subcultivated in vitro by means of GS interfering and controlled experiment. Methods : The 3^rd passage chondrocytes were divided into the blank group, different concentration GS groups passaged to 4^th generation, the control group of the 2nd passage. The chondrocytes of different groups were detected with the method of histochemistry for S - A - β - gal, for cell life cycle and proliferation index with flow eytometry, for the content and constructure of GAG of ECM with alcian blue test, and for type 11 collagen and Aggrecan with RT - PCR. A molecular biology study was also conducted on GS'preventive role against chondrocyte senescence. Results : GS can significantly inhibit chontrocyte's expression of S -A -β -gal, promote chontrocyte's proliferating( P 〈 0.01 ), reduce cell content in G1 phase, increase coment of GAG, and enchance the expression of type Ⅱ collagen and Aggrecan of ECM ( P 〈0.01 ). Conclusion: GS can significantly oppose rat chondrocyte senescence occurred in subcultivation.
Keywords:Glucosamin sulphat(GS)  Rat  Chondrocyte  Senenscence  
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