FGF2的生物信息学分析及其在低氧神经保护中的作用研究*

目的: 观察FGF2在低氧及低氧预适应条件下小鼠海马神经元细胞中的表达变化,探究FGF2是否参与低氧耐受神经保护作用。方法: 使用生物信息学方法进行搜索和鉴定FGF2基因家族成员。在细胞层面建立低氧和低氧预适应模型,通过Western-blot、qRT-PCR检测细胞中FGF2表达情况。结果: FGFs家族分为7个亚家族,其中FGF2含有一个由17个氨基酸组成的FGF受体结合域,能够与特定的FGF受体结合进行信号的转导。在细胞层面,相比于空白对照组(C),低氧组(H)和低氧预适应组(HPC)FGF2在mRNA水平和蛋白水平的表达均有所下降(P＜0.05),而与H组相比,HPC组FGF2 mRNA水平和蛋白水平的表达有所升高(P＜0.05)。结论: FGF参与低氧预适应的神经保护作用。

Bioinformatics analysis of FGF2 and its role in hypoxic neuroprotection

Objective: To observe the expression changes of FGF2 in mouse hippocampal neurons with hypoxia and hypoxia preconditioning, and to explore whether FGF2 participates in the neuroprotection of hypoxia tolerance. Methods: Bioinformatics methods were used search and identify FGF2 gene family members. At the cellular level, establish hypoxia and hypoxic preconditioning models, and detect FGF2 expression in cells by Western-blot and qRT-PCR. Results: The FGFs family is divided into 7 sub-families, among which FGF2 contains an FGF receptor binding domain composed of 17 amino acids, which can bind to specific FGF receptors for signal transduction. At the cell level, compared with control group, the expression of FGF2 mRNA and protein in H group and HPC group was significantly decreased (P＜0.05), while compared with H group, the expression of FGF2 mRNA and protein of HPC group was increased (P＜0.05). Conclusion: FGF participates in the neuroprotective effect of hypoxic preconditioning.