中西药联合对胃癌SGC-7901/ADR细胞MRP介导的耐药性研究

目的：探讨中西药联合逆转胃癌多药耐药亚系SGC-7901/ADR细胞MRP介导的耐药性作用。方法：以SGC-7901/ADR细胞为靶细胞,以不加药组为阴性对照组,5-FU组、斑贞1号组、班贞1号＋5-FU组、班贞1号＋5-FU＋TMP组为实验组,采用半定量RT-PCR法检测SGC-7901/ADR细胞在不同药物作用下MRP mRNA的表达情况。结果：5-FU组与阴性对照组比较,耐药细胞MRP mRNA表达差异无统计学意义（P〉0.05）,提示MRP参与SGC-7901/ADR细胞对5-FU的多药耐药性;其他实验组组间比较及与阴性对照组比较,MRP mRNA表达差异均有统计学意义（P〈0.01）,中西药联合组（斑贞1号＋5-FU＋TMP）MRP mRNA表达量最低（仅为0.07）。结论：中西药联合可以克服SGC-7901/ADR细胞由MRP介导的多药耐药性,为当前胃癌的治疗提供了新的理论依据。

Research on MRP Mediated Multi-drug Resistance in Gastric Carcinoma SGC-7901/ADR Cells by Combination of Modern Medicine and Traditional Chinese Medicine

Objective： To investigate the effects of combination of modern medicine and traditional Chinese medicine on MRP mediated multi-drug resistance in gastric carcinoma SGC-7901/ADR cells.Methods： The SGC-7901/ADR cells were regarded as target cells,no medicine added group were considered as negative control,5-FU,Banzhen-1,Banzhen-1＋5-FU,Banzhen-1＋5-FU＋TMP,were added to the target cells,respectively,and were considered as experimental groups.The expression of MRP mRNA in the SGC-7901/ADR cells was detected by the semi-quantitative RT-PCR.Results： No significant difference in the expression of MRP mRNA was detected between the 5-FU group and the negative controls（P0.05）,suggesting MRP was involved in 5-FU resistance in SGC-7901/ADR cells.However,the significant difference in the expression of MRP mRNA was seen between the other experimental groups and the negative controls,as well as among the different experimental groups（P﹤0.01）.The expression in the group of Banzhen-1＋5-FU＋TMP was the lowest（only 0.07）.Conclusion： The combination of modern medicine and traditional Chinese medicine could overcome the problem of MRP mediated multi-drug resistance in gastric carcinoma SGC-7901/ADR cells,which might supply a new theoretical basis for the treatment of gastric cancer.