血管生成抑制剂联合分化诱导剂治疗结肠癌肝脏转移的实验研究

目的:实验通过建立人LOVO细胞结肠癌裸鼠肝脏转移瘤模型,研究血管生成抑制剂(TNP-470)联合分化诱导剂(RA)抗结肠癌转移的效应,二者是否具有协同效果.方法:建立人LOVO细胞结肠癌裸鼠肝脏转移瘤模型,随机分为4组,模型建立后分组治疗,至治疗结束时处死裸鼠,检测肝脏转移率和肝转移结节数及肝脏转移瘤组织中血管内皮生...

Experimental Study of the Therapy Effects of Angiogenesis Inhibitor and Differentiation Inducer on Human Colon Cancer

Objective： To study the anti-cancer metastasis effects of angiogenesis inhibitor TNP-470 combined with differention inducer RA by human colon cancer liver metastasis into nude mice.Method： Liver metastases tumor into nude mice with colon cancer of LOVO cell was established.The nude mice were divided into 4 groups at random.Treated respectively from one day after implanted and metastased,all the nude mice were sacrificed by the end of treatment,then observed the liver metastasis rate and tumor foci as well as expression of VEGF in the liver metastatic tumors were counted.Result： TNP-470 in combination with RA and TNP-470 alone display a significant inhibitory effect on liver metastasis compared to that of the control group（P0.05）,the combination group was different from RA group on liver metastasis（P0.05）.The expression of VEGF in the liver metastasis tumors was clearly inhibited by TNP-470 in combination with RA and RA alone compared to that of the control（P0.05）.Conclusion： The angiogenesis inhibitor TNP-470 in combination with differention inducer RA might has additive effect on the liver metastasis in nude mice.RA also could inhibit carcinoma cell proliferation by inducing apoptosis of colon cancer cell,inhibiting expression of VEGF.