CK 7CK20 CA125及TTF-1在液基细胞学腺癌细胞中的表达及意义分析

目的:研究CK7、CK20、CA125及TTF-1在液基细胞学腺癌细胞中的表达情况并分析其临床意义.方法:选取从2015年2月至2016年3月,我院病理科经组织学确诊为腺癌的浆膜腔积液标本137例,利用液基薄层细胞学制片技术以及免疫细胞化学染色方法,观察不同组织来源腺癌细胞的CK7、CK20、CA125及TTF-1等抗体的表达情况.结果:CK7在肺腺癌的阳性率100%,卵巢浆液性癌大部分表达,肠道腺癌个别阳性;CK20在肠道腺癌中的表达阳性率为88.89%(32/36),在肺腺癌及卵巢浆液性癌中未见表达;CA125在卵巢浆液性癌中表达率为70.45%(31/44),在肺腺癌以及肠道腺癌中表达较少;TTF-1在肺腺癌中的表达阳性率为80.70%(46/57),在卵巢浆液性癌及肠道腺癌中未见表达,差异均有统计学意义(均P<0.05).低分化肺腺癌中TTF-1表达阳性率显著低于中高分化,差异均有统计学意义(均P<0.05).低分化卵巢浆液性癌中CA125表达阳性率显著低于中高分化,差异有统计学意义(P<0.05).低分化肠道腺癌中CK20表达阳性率显著低于中高分化,差异有统计学意义(P<0.05).结论:CK7、CK20、CA125及TTF-1在液基细胞学腺癌细胞中的表达情况有利于鉴别浆膜腔积液中来自肺、卵巢以及肠道的腺癌.

Expression and Significance of CK7 CK20 CA125 and TTF-1in Liquid Based Cytology Adenocarcinoma Cells

Objective:To study the expression of CK7, CK20, A125 and TTF-1 in liquid based cytolo-gy adenocarcinoma cells, and analyze its clinical significance. Methods:137 cases serous effusion specimens of adenocarcinoma diagnosed in our hospital from February 2015 to March 2016 were selected. Using the liquid based cytology production technology and immunocytochemical staining method, the different tissue derived adenocarcinoma cells CK7, CK20, CA125 and TTF-1 antibodies were observed. Results:The positive rate of CK7100% in lung adenocarcinoma, most ovarian serous carcinoma express, intestinal adenocarcinoma indi-vidual positive;CK20 expression in intestinal adenocarcinoma positive rate was 88.89% (32/36),There's no expression in lung adenocarcinoma; CA125 expression in ovarian serous adenocarcinoma rate was 70. 45%(31/44), in lung adenocarcinoma and expression in ovarian serous carcinoma was less;The vera TTF-1 ex-pression in lung adenocarcinoma positive rate was 80.70% (46/57), in ovarian serous carcinoma, and no ex-pression in intestinal adenocarcinoma, differences were statistically significant ( all P <0.05) . Poorly differen-tiated lung adenocarcinoma express the vera.TTF-1 positive rate was significantly lower than the high differen-tiation, differences were statistically significant ( all P < 0.05) . Poorly differentiated CA125 expression in o-varian serous carcinoma positive rate was significantly lower than the high differentiation, the difference was statistically significant (P < 0.05). Poorly differentiated CK20 expression in intestinal adenocarcinoma were significantly lower than the high differentiation, the difference was statistically significant (P <0.05).Conclu-sion:The expression of CK7, CK20, CA125 and TTF-1 in the liquid based cytology adenocarcinoma cells is beneficial to identify the serous cavity effusion from lung, ovary and intestinal adenocarcinoma.