SiO_2粉尘刺激对NR8383细胞分泌炎症因子的影响

目的:比较不同剂量Si O2粉尘刺激NR8383细胞后TNF-α,IL-1β,TGF-β基因表达水平变化。方法:细胞接受不同剂量粉尘刺激后,采用MTT法检测各组细胞活力的改变,筛选出细胞存活率比较高的剂量。细胞随机分为四组,即对照组,10μg/cm2组、20μg/cm2组和40μg/cm2组,接受粉尘刺激12 h后,采用荧光定量PCR法测定各组TNF-α,IL-1β,TGF-β基因表达水平。结果:随着染尘剂量的提高,细胞存活率逐渐降低,80μg/cm2组细胞存活率最低。实时荧光定量PCR显示10μg/cm2组、20μg/cm2组和40μg/cm2组TNF-α,IL-1β,TGF-β基因表达水平均高于对照组,差异具有统计学意义(P<0.05)。随着染尘剂量的递增,TNF-α,IL-1β,TGF-β的mRNA相对表达水平逐渐增高。结论:粉尘刺激可以引起大鼠肺泡巨噬细胞分泌炎症因子增加,呈现一定的剂量效应关系。

The effect on secretion of inflammatory cytokines of NR8383 cells stimulated by silica dust

Objective: To compare the gene expression of TNF-α,IL-1β and,TGF-β in NR8383 cells stimulated by different doses of Si O2 dust. Methods: After the cells were stimulated by different doses Si O2 dust,MTT methods was used to assay the cell viability,and doses with high survival rate were selected. The cells were divided into four groups: control group,10μg / cm2 group,20μg /cm2 group and 40μg / cm2 group. 12 hours after stimulated by dust,real time PCR method was used to detect the gene expression of TNF-α,IL-1β and,TGF-β. Results: With the increasing of exposure dose,cell survival rate decreased. 80μg /cm2 group has the lowest survival rate. The gene expression of 10μg / cm2 group,20μg / cm2 group and 40μg / cm2 group were significantly higher than that of the control group. There are statistically differences.( P < 0. 05). With the increasing of exposure dose,the gene expression level of mRNA of TNF-α,IL-1β and TGF-β has an increasing trend accordingly. Conclusion: The silica dust could cause rat alveolar macrophages to secret inflammatory cytokines with a dose-response relationship.