| 车前子超微速溶饮片中京尼平苷酸与毛蕊花糖苷的含量测定 |
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| 引用本文： | 陈乐,谢玲,熊学敏.车前子超微速溶饮片中京尼平苷酸与毛蕊花糖苷的含量测定[J].江西中医学院学报,2013(5):56-58. |
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| 作者姓名： | 陈乐 谢玲 熊学敏 |
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| 作者单位： | [1]江西省中医药研究院,南昌330077 [2]江西中医学院,南昌330004 |
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| 摘 要： | 目的：建立一种同步测定车前子超微速溶饮片中京尼平苷酸与毛蕊花糖苷含量的高效液相色谱方法。方法：色谱柱为HypersilODS2C18柱（4．6mm×250mm，5p,m），流速：1．0mL/min，检测波长：254nm，柱温：35℃。结果：京尼平苷酸与毛蕊花糖苷的线性范围分别为59．88μg/mL～159．68μg/mL（r=0．9999）和91．26μg/mL～243．36μg/mL（r=0．9999）；平均加样回收率分别为100．5％（RSD：1．1％，n=6）和99．7％（RSD=1．3％，n=6）。结论：本方法操作简便，测定结果准确，重复性好，可作为该产品质控的方法。
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| 关 键 词： | 京尼平苷酸 毛蕊花糖苷 HPLC |
The Determination of Geniposidic Acid and Verbascoside in Plantain Seed Ultra Instant Pieces |
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| CHEN Le,XIE Ling,XIONG Xue-min.The Determination of Geniposidic Acid and Verbascoside in Plantain Seed Ultra Instant Pieces[J].Journal of Jiangxi College of Traditional Chinese Medicine,2013(5):56-58. |
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| Authors: | CHEN Le XIE Ling XIONG Xue-min |
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| Institution: | 1. Jiangxi Chinese Traditional Medicine institute of Research, Nanchang , 330077, China; 2. Jiangxi Chinese Traditional Medicine University, Nanchang, 330004, China.) |
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| Abstract: | Objective： To establish a method for the determination of geniposidic acid and verbascoside in superfine instant pieces of Se men Plantaginis by HPLC. Methods：The HPLC analysis was performed on a Hypersil ODS2 Clscolumn （4.6mm x 250mm, 5μm） ,The flow rate was 1.0mL/min, the detection wave -length was 254nm, the column temperature was 30℃. Results： The linear ranges of ge- niposidic acid and verbascoside were59.88 ～ 159. 68μg . mL^-1 （ r = 0. 9999 ） and91.26μg/mL ～ 243.36μg/mL （ r = 0.9999） ;The av- erage recoveries were 100.5% （RSD = 1.1% ,n =6） and 99.7% （RSD = 1.3% ,n =6）. Conclusion：The method is convenient,rapid and accurate. It can be used in the quality control of superfine instant pieces of Semen Plantaginis . |
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| Keywords: | Geniposidic Acid Verbascoside HPLC |
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