| Eotaxin/CCR3在子宫内膜异位症不孕小鼠的表达及中药莪棱胶囊的干预作用 |
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| 引用本文： | 孙静,张维彬,韩凌,黄海定,邓时贵.Eotaxin/CCR3在子宫内膜异位症不孕小鼠的表达及中药莪棱胶囊的干预作用[J].江西中医学院学报,2011,23(4):61-63. |
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| 作者姓名： | 孙静 张维彬 韩凌 黄海定 邓时贵 |
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| 作者单位： | 1. 广州中医药大学第二临床医学院中心实验室 广州510006
2. 南方医科大学北滘医院 顺德528000 |
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| 基金项目： | 广州中医药大学创新基金，广东省科技厅课题，广州中医药管理局 |
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| 摘 要： | 目的:通过建立子宫内膜异位症(EMS)导致不孕症小鼠模型,观察比较趋化因子EOTAXIN及其受体CCR3在子宫内膜异位症(内异症)怀孕鼠以及不孕鼠之间的差异,分析EOTAXIN/CCR3基因对内异症不孕的影响,并探讨中药莪棱胶囊的干预作用。方法:建立EMS小鼠模型,同时皮下注射乙烯雌酚。观察24周后,进行同笼交配以计算该模型不孕率,同时区分怀孕鼠以及不孕鼠。对不孕鼠口服给予莪棱胶囊进行治疗,4周后取材,采用荧光定量PCR法分别观察正常鼠、怀孕鼠、不孕给药鼠、不孕给水鼠子宫内膜中Eotaxin/CCR3的表达情况。结果:假手术组小鼠子宫内膜Eotaxin表达,以及CCR3基因表达均较低。EMS模型怀孕鼠子宫内膜Eotaxin/CCR3基因表达明显升高。但EMS模型不孕鼠子宫内膜Eotaxin,以及CCR3基因表达较怀孕鼠更加明显。在不孕鼠中,给予莪棱胶囊的小鼠,子宫内膜Eotaxin表达以及CCR3表达均较给水组降低。结论:EOTAXIN/CCR3可能直接参与了子宫内膜异位症导致的不孕症的病理过程。莪棱胶囊可直接下调不孕鼠子宫内膜EOTAX-IN/CCR3表达,而起到缓解炎症,改善内异症作用的。
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| 关 键 词： | 子宫内膜异位 EOTAXIN/CCR3 莪棱胶囊 |
Eotaxin/CCR3 Expression in Endometriosis Infertility Mice and the Invention of Traditional Chinese Medicine Erling Capsules |
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| SUN Jing,ZHANG Wei-Bin,HAN Ling,HUANG Hai-ding,DENG Shi-gui.Eotaxin/CCR3 Expression in Endometriosis Infertility Mice and the Invention of Traditional Chinese Medicine Erling Capsules[J].Journal of Jiangxi College of Traditional Chinese Medicine,2011,23(4):61-63. |
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| Authors: | SUN Jing ZHANG Wei-Bin HAN Ling HUANG Hai-ding DENG Shi-gui |
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| Institution: | SUN Jing1,ZHANG Wei-Bin2,HAN Ling1,HUANG Hai-ding1,DENG Shi-gui1 1.Guangzhou University of Chinese Medicine,Second Affiliated Hospital Guangdong,Guangzhou,510006,2.Beijiao hospital affiliated to Southern Medical University,Guangdong,Shunde 528000 |
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| Abstract: | Objective: To establish endometriosis(EMS) infertility in mice models,observe and compare the difference of expression of chemokine EOTAXIN and its receptor CCR3 between endometriosize pregnant and infertile mice,analyze the effect of EOTAXIN/CCR3 gene expression on endometriosis infertility,and to explore Traditional Chinese Medicine Erling capsule intervention.Methods: Established an EMS mouse model,while subcutaneous injection of diethylstilbestrol.Observed after 24 weeks,mice were mated within the same ... |
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| Keywords: | endometriosis EOTAXIN/CCR3 Eling capsule |
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