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| Expression of nucleostemin in prostate cancer and its effect | |
| 作者姓名： | Liu RL Zhang ZH Zhao WM Wang M Qi SY Li J Zhang Y Li SZ Xu Y |
| 作者单位： | LIU Ran-lu,ZHANG Zhi-hong,ZHAO Wei-ming,WANG Meng,QI Shi-yong,LI Jing,XU Yong(Department of Urology,Second Hospital of Tianjin Medical University,Tianjin Institute of Urology,Tianjin 300211,China);ZHANG Yue,LI Sheng-zhi(Tianiin Institute of Urology,Tianiin 300211,China) |
| 基金项目： | Tianjin Municipal Commission of Education，天津市自然科学基金 |
| 摘 要： | Background Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis. Methods Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nucleostemin mRNA and protein expression were measured by RT-PCR and Western blot. Immunohistochemistry was also used to detect the nucleostemin protein expression in prostate cancer tissues and PC-3 cells. A nucleostemin specific, short hairpin RNA, expression plasmid was used to transfect PC-3 cells. The changes of nucleostemin gene were detected and the oroliferative capacitv of the cells was determined. Results Nucleostemin was highly expressed in prostate cancer tissues and cell lines. Nucleostemin expression level in the silencer group PC-3 cells remarkably reduced. The proliferation rate of silencer group PC-3 cells decreased and the percentage of G1 stage cells increased. The neoplasm forming capacity in nude mice of the silencer group PC-3 cells decreased significantly. Conclusions Nucleostemin is highly expressed in prostate cancer tissues and cell lines. The proliferative capacity of PC-3 cells is remarkably reduced after silencing nucleostemin gene expression. |
| 关 键 词： | 前列腺癌 RNA干扰技术 病理机制 临床表现 |
Expression of nucleostemin in prostate cancer and its effect |
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| Liu RL,Zhang ZH,Zhao WM,Wang M,Qi SY,Li J,Zhang Y,Li SZ,Xu Y.Expression of nucleostemin in prostate cancer and its effect[J].Chinese Medical Journal,2008,121(4):299-304. | |
| Authors: | Liu Ran-lu Zhang Zhi-hong Zhao Wei-ming Wang Meng Qi Shi-yong Li Jing Zhang Yue Li Sheng-zhi Xu Yong |
| Institution: | 1. Department of Urology,Second Hospital of Tianjin Medical University,Tianjin Institute of Urology,Tianjin 300211,China 2. Tianiin Institute of Urology,Tianiin 300211,China |
| Abstract: | Background Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis.Methods Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nucleostemin mRNA and protein expression were measured by RT-PCR and Western blot. Immunohistochemistry was also used to detect the nucleostemin protein expression in prostate cancer tissues and PC-3 cells. A nucleostemin specific, short hairpin RNA, expression plasmid was used to transfect PC-3 cells. The changes of nucleostemin gene were detected and the proliferative capacity of the cells was determined.Results Nucleostemin was highly expressed in prostate cancer tissues and cell lines. Nucleostemin expression level in the silencer group PC-3 cells remarkably reduced. The proliferation rate of silencer group PC-3 cells decreased and the percentage of G1 stage cells increased. The neoplasm forming capacity in nude mice of the silencer group PC-3 cells decreased significantly.Conclusions Nucleostemin is highly expressed in prostate cancer tissues and cell lines. The proliferative capacity of PC-3 cells is remarkably reduced after silencing nucleostemin gene expression. |
| Keywords: | nucleostemin prostatic neoplasms PC-3 RNA interference |
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