The relationship between MTHFR gene polymorphisms, plasma homocysteine levels and diabetic retinopathy in type 2 diabetes mellitus |
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Authors: | Sun Jiazhong Xu Yancheng Zhu Yilian Lu Hongyun Deng Haohua Fan Youyun Sun Suxin Zhang Ying |
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Institution: | Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China |
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Abstract: | Objective To evaluate the role of methylenetetrahydrofolate reductase (MTHFR) gene polymor phisms and plasma homocysteine levels in patients with type 2 diabetes mellitus and diabetic retinopathy (DR).
Methods Total of 208 patients with type 2 diabetes mellitus and 57 controls were recruit ed into the study. MTHFR genetic C677T polymorphisms were determined by PCR- RF LP. Plasma total homocysteine levels were measured using high- performance liqu id chromatography (HPLC) with fluorescence detection.
Results The frequencies of MTHFR TT homogeneous type, CT heterogeneous type and allele T (28.18%, 41.82%, 49.09%) were significantly higher in the type 2 diabetes me llitus with diabetic retinopathy group than those without retinopathy (18.37%, 29.59%, 33.16%) and those of controls (17.54%, 28.07%, 31.58%). The presen ce of the T allele appeared to have a strong association with the development of diabetic retinopathy. The odds ratio was 1.94 with a 95% confidence interval of 1.31-2.88. Moreover, plasma homocysteine levels were remarkably higher in patients with TT or CT genotype than in patients with the CC genotype.
Conclusion MTHFR gene C677T mutation associated with a predisposition to increased plasma h omocysteine levels may be considered as a genetic risk factor for diabetic micro angiopathy (such as DR) in Chinese patients with type 2 diabetes mellitus. |
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Keywords: | diabetic retinopathy methylenetetrahydrofolate reductase gene homocysteine |
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