| Effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with IL-2 or TNF-α |
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| 作者姓名： | Zhan Y Wang Y Wei L Chen H |
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| 作者单位： | Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing 100730, China;Heptatology Institute,People’s Hospital, Beijing University, Beijing 100044, China;Heptatology Institute,People’s Hospital, Beijing University, Beijing 100044, China;Heptatology Institute,People’s Hospital, Beijing University, Beijing 100044, China |
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| 基金项目： | ThisworkwassupportedbyagrantfromNational 863Program(No 2 0 0 1AA2 1715 1) |
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| 摘 要： | Objective To study the effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with interleukin-2 (IL-2 ) or tumor necrosis factor-α (TNF-α).
Methods Hepatic stellate cells were isolated from male Sprague-Dawley rats by using modified Friedman’s method. Using the isolated cells cultured and treated with IL-2 or TNF-α, we studied the effects of vitamin E on their proliferation and collagen synthesis through an (3)H-thymidine and (3)H-proline incorporation assay, as well as through observation of these cells under a contrary phase microscope.
Results Adding IL-2 increased the both proliferation and collagen synthesis of hepatic stellate cells. Their proliferation was also increased by the addition of TNF-α, although it decreased collagen synthesis. Vitamin E had marked inhibitory effects on the ability of cells treated with IL-2 or TNF-α to reproduce or synthesize collagen.
Conclusion Vitamin E can inhibit the proliferation and collagen synthesis of hepatic stellate cells. It is possible that vitamin E affects liver fibrosis through these activities.
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| 关 键 词： | hepatic stellate cells vitamin E tumor necrosis factor-α. interleukin-2 |
Effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with IL-2 or TNF-alpha |
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| Zhan Y,Wang Y,Wei L,Chen H.Effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with IL-2 or TNF-alpha[J].Chinese Medical Journal,2003,116(3):472-474. |
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| Authors: | Zhan Yutao Wang Yu Wei Lai Chen Hongsong |
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| Institution: | 1. Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing 100730, China
2. Heptatology Institute, People's Hospital, Beijing University, Beijing 100044, China |
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| Abstract: | OBJECTIVE: To study the effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with interleukin-2 (IL-2) or tumor necrosis factor-alpha (TNF-alpha). METHODS: Hepatic stellate cells were isolated from male Sprague-Dawley rats by using modified Friedman's method. Using the isolated cells cultured and treated with IL-2 or TNF-alpha, we studied the effects of vitamin E on their proliferation and collagen synthesis through an (3)H-thymidine and (3)H-proline incorporation assay, as well as through observation of these cells under a contrary phase microscope. RESULTS: Adding IL-2 increased the both proliferation and collagen synthesis of hepatic stellate cells. Their proliferation was also increased by the addition of TNF-alpha, although it decreased collagen synthesis. Vitamin E had marked inhibitory effects on the ability of cells treated with IL-2 or TNF-alpha to reproduce or synthesize collagen. CONCLUSION: Vitamin E can inhibit the proliferation and collagen synthesis of hepatic stellate cells. It is possible that vitamin E affects liver fibrosis through these activities. |
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| Keywords: | hepatic stellate cells vitamin E tumor necrosis factor-α Interleukin-2 |
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