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Decreased expression of AQP1 and AQP5 in acute injured lungs in rats
作者姓名:Jiao G  Li E  Yu R
作者单位:中国医科大学第一临床学院呼吸疾病研究所,沈阳,110001
基金项目:ThisprojectwassupportedbytheNationalNaturalScienceFoundationof China (No 3 9870 3 3 8)
摘    要:Objective To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs. Methods The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity- purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS). Results Immunolabelling showed AQP1 was stained primarily in the microvascular endothelia of normal lungs, while AQP5 was expressed in type Ⅰ pneumocytes. Immunohistochemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged. Conclusion The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.

关 键 词:内毒素  呼吸膜  水通道蛋白1  水通道蛋白5  急性肺损伤  免疫组织化学

Decreased expression of AQP1 and AQP5 in acute injured lungs in rats
Jiao G,Li E,Yu R.Decreased expression of AQP1 and AQP5 in acute injured lungs in rats[J].Chinese Medical Journal,2002,115(7):963-967.
Authors:Jiao Guangyu  Li Erran  Yu Runjiang
Institution:Institute of Respiratory Disease, The First Clinical Hospital, China Medical University, Shenyang 110001, China;Institute of Respiratory Disease, The First Clinical Hospital, China Medical University, Shenyang 110001, China;Institute of Respiratory Disease, The First Clinical Hospital, China Medical University, Shenyang 110001, China
Abstract:OBJECTIVE: To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs. METHODS: The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS). RESULTS: Immunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged. CONCLUSION: The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.
Keywords:aquaporin 1  aquaporin 5  acute lung injury  steroid
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