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Detection of Epstein-Barr virus DNA in plasma/serum: a useful serological indicator for diagnosis of nasopharyngeal carcinoma
作者姓名:Mai S  Zong Y  Zhang M  Zhong B  Lin S
作者单位:中山大学中山医学院病理学教研室,广州,510089
基金项目:National Natural Science Foundation of China,No. 39730200-Ⅱ;
摘    要:Objective To compare the detection rates of Epstein-Barr virus (EBV) DNA in the serum/plasma between apparently healthy adults (AHAs) and nasopharyngeal carcinoma (NPC) patients in attempt to evaluate the efficiency of EBV DNA assay for serodiagnosis of NPC.Methods The plasma and serum were obtained from 58 AHAs and 66 untreated NPC patients. EBV DNA W-fragment was detected using nested ploymerase chain reaction (PCR). Immunoenzymatic assay for titration of IgA-VCA was also adopted. Results EBV DNA detection rate (84.85%) in the plasma/serum of 66 NPC patients was significantly higher than that (10.34%) in 58 AHAs. The sensitivity of plasma/serum EBV DNA assay (0.8485) was higher than that (0.8030) of titrating IgA-VCA (positive criterion≥1∶40) though the specificities of these two tests were the same (0.8966). The correct rate, predictive value of a positive test, and Odds ratio of dual positivity (0.8387, 0.9792 and 141.0, respectively) were higher than those of single positivity either to plasma/serum EBV assay (0.5242, 0.7333 and 1.1423, respectively) or to IgA-VCA≥1∶40 test (0.4839, 0.5385 and 1.0480, respectively). Conclusion The EBV DNA detection in the plasma/serum using nested PCR may be a useful indicator for serodiagnosis of NPC.

关 键 词:鼻咽癌  鼻咽肿瘤  血清学检测  DNA检测  Epstein-Barr病毒

Detection of Epstein-Barr virus DNA in plasma/serum: a useful serological indicator for diagnosis of nasopharyngeal carcinoma
Mai S,Zong Y,Zhang M,Zhong B,Lin S.Detection of Epstein-Barr virus DNA in plasma/serum: a useful serological indicator for diagnosis of nasopharyngeal carcinoma[J].Chinese Medical Journal,2002,115(12):1895-1897.
Authors:Mai Shijuan  Zong Yongsheng  Zhang Min  Zhong Biling  Lin Suxia
Institution:Department of Pathology, Sun Yat-Sen Medical College, Sun Yat-Sen University, Guangzhou 510089, China;Department of Pathology, Sun Yat-Sen Medical College, Sun Yat-Sen University, Guangzhou 510089, China;Department of Pathology, Sun Yat-Sen Medical College, Sun Yat-Sen University, Guangzhou 510089, China;Department of Pathology, Sun Yat-Sen Medical College, Sun Yat-Sen University, Guangzhou 510089, China;Department of Pathology, Sun Yat-Sen Medical College, Sun Yat-Sen University, Guangzhou 510089, China
Abstract:OBJECTIVE: To compare the detection rates of Epstein-Barr virus (EBV) DNA in the serum/plasma between apparently healthy adults (AHAs) and nasopharyngeal carcinoma (NPC) patients in attempt to evaluate the efficiency of EBV DNA assay for serodiagnosis of NPC. METHODS: The plasma and serum were obtained from 58 AHAs and 66 untreated NPC patients. EBV DNA W-fragment was detected using nested ploymerase chain reaction (PCR). Immunoenzymatic assay for titration of IgA-VCA was also adopted. RESULTS: EBV DNA detection rate (84.85%) in the plasma/serum of 66 NPC patients was significantly higher than that (10.34%) in 58 AHAs. The sensitivity of plasma/serum EBV DNA assay (0.8485) was higher than that (0.8030) of titrating IgA-VCA (positive criterion >/= 1:40) though the specificities of these two tests were the same (0.8966). The correct rate, predictive value of a positive test, and Odds ratio of dual positivity (0.8387, 0.9792 and 141.0, respectively) were higher than those of single positivity either to plasma/serum EBV assay (0.5242, 0.7333 and 1.1423, respectively) or to IgA-VCA >/= 1:40 test (0.4839, 0.5385 and 1.0480, respectively). CONCLUSION: The EBV DNA detection in the plasma/serum using nested PCR may be a useful indicator for serodiagnosis of NPC.
Keywords:nasopharyngeal neoplasm  Epstein-Barr virus  DNA  serum  plasma
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