首页 | 本学科首页   官方微博 | 高级检索  
检索        

高脂饮食逆转十二指肠空肠旁路术对2型糖尿病大鼠糖耐量的改善作用的研究
引用本文:刘少壮,孙栋,张光永,王磊,刘腾,孙宇,李明霞,胡三元.高脂饮食逆转十二指肠空肠旁路术对2型糖尿病大鼠糖耐量的改善作用的研究[J].中华医学杂志(英文版),2012,125(5):912-919.
作者姓名:刘少壮  孙栋  张光永  王磊  刘腾  孙宇  李明霞  胡三元
作者单位:Department of General Surgery, Qilu Hospital of Shandong University,Department of General Surgery, Qilu Hospital of Shandong University,Department of General Surgery, Qilu Hospital of Shandong University,Department of General Surgery, Qilu Hospital of Shandong University,Department of General Surgery, Qilu Hospital of Shandong University,Department of General Surgery, Qilu Hospital of Shandong University,Experimental Center, School of Chemistry and Chemical Engineering, Shandong University,Department of General Surgery, Qilu Hospital of Shandong University
基金项目:grants,from,the,National,Natural,Science,Foundation,of,China,the,Specialized,Research,Fund,for,the,Doctoral,Program,of,Higher,Education,the,Graduate,Independent,Innovation,Foundation,of,Shandong,University
摘    要:
Background  Bariatric surgery offers successful resolution of type 2 diabetes mellitus (T2DM). However, recurrence of T2DM has been observed in a number of patients with initial resolution after bariatric surgery. This study aimed to induce reversal of the improvement of diabetes in T2DM rats after duodenal-jejunal bypass (DJB), and identify the effects of weight changes and gut hormones that might be involved.
Methods  DJB surgery was performed in two T2DM rat models (n=20 for each group): non-obese Goto-Kakizaki (GK) rats, and moderately-obese T2DM rats induced by a combination of a high-fat diet (HFD) and low-dose streptozotocin (HS rats). The controls were sham-operated and non-treated rats. All rats were then randomly divided into HFD- and low-fat diet (LFD)-fed groups. Glucose tolerance, insulin tolerance, glucose-stimulated insulin, glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) secretion, food intake and body weight were measured and compared with controls.
Results  DJB surgery resulted in a significant improvement in glucose tolerance in both GK and HS rats fed with either HFD or LFD. In contrast to LFD-fed rats, improved glucose tolerance was impaired in GK and HS rats fed with an HFD, accompanied by re-impairment of insulin tolerance and failure in enhancement of insulin secretion. There was no significant difference in food intake and body weight between DJB-operated and control rats, and between HFD- and LFD-fed rats. Glucose-stimulated GLP-1 and PYY levels were significantly increased after DJB surgery; however, they were not significantly different between HFD- and LFD-fed rats.
Conclusion  An HFD reverses the improvement in glucose tolerance induced by DJB surgery in T2DM rats, primarily ascribing to the re-impairment of insulin sensitivity, but does not change body weight, GLP-1 and PYY levels.
 


关 键 词:duodenal-jejunal  bypass    type  2  diabetes  mellitus    recurrence    glucagon-like  peptide-1

A high-fat diet reverses improvement in glucose tolerance induced by duodenal-jejunal bypass in type 2 diabetic rats
Liu Shao-zhuang,Sun Dong,Zhang Guang-yong,Wang Lei,Liu Teng,Sun Yu,Li Ming-xia,Hu San-yuan.A high-fat diet reverses improvement in glucose tolerance induced by duodenal-jejunal bypass in type 2 diabetic rats[J].Chinese Medical Journal,2012,125(5):912-919.
Authors:Liu Shao-zhuang  Sun Dong  Zhang Guang-yong  Wang Lei  Liu Teng  Sun Yu  Li Ming-xia  Hu San-yuan
Institution:Department of General Surgery, Qilu Hospital of Shandong University, Experimental Center, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250012, China.
Abstract:Background Bariatric surgery offers successful resolution of type 2 diabetes mellitus (T2DM).However,recurrence of T2DM has been observed in a number of patients with initial resolution after bariatric surgery.This study aimed to induce reversal of the improvement of diabetes in T2DM rats after duodenal-jejunal bypass (DJB),and identify the effects of weight changes and gut hormones that might be involved.Methods DJB surgery was performed in two T2DM rat models (n=20 for each group):non-obese Goto-Kakizaki (GK) rats,and moderately-obese T2DM rats induced by a combination of a high-fat diet (HFD) and low-dose streptozotocin (HS rats).The controls were sham-operated and non-treated rats.All rats were then randomly divided into HFD- and low-fat diet (LFD)-fed groups.Glucose tolerance,insulin tolerance,glucose-stimulated insulin,glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) secretion,food intake and body weight were measured and compared with controls.Results DJB surgery resulted in a significant improvement in glucose tolerance in both GK and HS rats fed with either HFD or LFD.In contrast to LFD-fed rats,improved glucose tolerance was impaired in GK and HS rats fed with an HFD,accompanied by re-impairment of insulin tolerance and failure in enhancement of insulin secretion.There was no significant difference in food intake and body weight between DJB-operated and control rats,and between HFD- and LFD-fed rats.Glucose-stimulated GLP-1 and PYY levels were significantly increased after DJB surgery; however,they were not significantly different between HFD- and LFD-fed rats.Conclusion An HFD reverses the improvement in glucose tolerance induced by DJB surgery in T2DM rats,primarily ascribing to the re-impairment of insulin sensitivity,but does not change body weight,GLP-1 and PYY levels.
Keywords:duodenal-jejunal bypass  type 2 diabetes mellitus  recurrence  glucagon-like peptide-1
本文献已被 PubMed 等数据库收录!
点击此处可从《中华医学杂志(英文版)》浏览原始摘要信息
点击此处可从《中华医学杂志(英文版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号