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| Effects of magnesium sulfate on neuron apoptosis and expression of caspase- 3， bax and bcl-2 after cerebral ischemia-reperfusion injury | |
| 作者姓名： | Zhou H Ma Y Zhou Y Liu Z Wang K Chen G |
| 作者单位： | Department of Anesthesiology,the Second Affiliated Hospital,Medical College of Zhejiang University,Department of Emergency,the Second Affiliated Hospital,Medical College of Zhejiang University,Department of Anesthesiology,the Second Affiliated Hospital,Medical College of Zhejiang University,Department of Anesthesiology,the Second Affiliated Hospital,Medical College of Zhejiang University,Department of Respiration,the Second Affiliated Hospital,Medical College of Zhejiang University,Department of Anesthesiology,the Second Affiliated Hospital,Medical College of Zhejiang University Hangzhou 310009,China,Hangzhou 310009,China,Hangzhou 310009,China,Hangzhou 310009,China,Hangzhou 310009,China,Hangzhou 310009,China |
| 摘 要： | Objective To investigate the effects of magnesium sulfate on neuron apoptosis and the expressions of caspase-3,bax and bcl-2 after cerebral ischemia-reperfusion injury. Methods Thirty-six gerbils were randomly divided into three groups: Sham-operation group (So, n=12), ischemia-reperfusion group (I-R, n=12) and magnesium sulfate group (Ms, n=12). The neuron apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-fluorescence nick end labeling (TUNEL stain), and the protein expressions of caspase-3, bax and bcl-2 were detected by immunohistochemisty stain.Results Apoptotic neurons and the expressions of caspase-3 and bax were significantly increased in I-R and Ms groups, compared with those in So group. Apoptotic neurons and the expressions of bax and caspase-3 in Ms group were significantly less than those in I-R group. There was no significant difference in the expression of bcl-2 among three groups.Conclusions Magnesium sulfate decreases neuron apoptosis after cerebral ischemia-reperfusion injury, which may be related with the suppressed expressions of caspase-3 and bax. |
| 关 键 词： | magnesium sulfate · ischemia-reperfusion injury · apoptosis · caspase-3 · bax · bcl-2 |
Effects of magnesium sulfate on neuron apoptosis and expression of caspase-3, bax and bcl-2 after cerebral ischemia-reperfusion injury |
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| Zhou H,Ma Y,Zhou Y,Liu Z,Wang K,Chen G.Effects of magnesium sulfate on neuron apoptosis and expression of caspase-3, bax and bcl-2 after cerebral ischemia-reperfusion injury[J].Chinese Medical Journal,2003,116(10):1532-1534. | |
| Authors: | Zhou Haiyan Ma Yuefeng Zhou Yifeng Liu Ziming Wang Kai Chen Gang |
| Institution: | 1. Department of Anesthesiology, the Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China 2. Department of Emergency, the Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China 3. Department of Respiration, the Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China |
| Abstract: | Objective To investigate the effects of magnesium sulfate on neuron apoptosis and the expressions of caspase-3,bax and bcl-2 after cerebral ischemia-reperfusion injury. Methods Thirty-six gerbils were randomly divided into three groups: Sham-operation group (So,n=12),ischemia-reperfusion group (I-R,n=12) and magnesium sulfate group (Ms,n=12). The neuron apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-fluorescence nick end labeling (TUNEL stain),and the protein expressions of caspase-3,bax and bcl-2 were detected by immunohistochemisty stain.Results Apoptotic neurons and the expressions of caspase-3 and bax were significantly increased in I-R and Ms groups,compared with those in So group. Apoptotic neurons and the expressions of bax and caspase-3 in Ms group were significantly less than those in I-R group. There was no significant difference in the expression of bcl-2 among three groups.Conclusions Magnesium sulfate decreases neuron apoptosis after cerebral ischemia-reperfusion injury,which may be related with the suppressed expressions of caspase-3 and bax. |
| Keywords: | magnesium sulfate ischemia-reperfusion injury apoptosis caspase-3 bax bcl-2 |
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