Prediction of recurrence risk in early breast cancer using human epidermal growth factor 2 and cyclin A2 |
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Authors: | LI Hui-ping JI Jia-fu HOU Kuan-yong LEI Yu-tao ZHAO Hong-mei WANG Jing ZHENG Jie LIU Jian-ying WANG Mo-pei XIAO Yu WANG You-fan L You-yong SUN Yan |
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Institution: | 1. Department of Medical Oncology ,Peking University Third Hospital, Beijing 100191, China 2. Department of Surgery, Peking University School of Oncology,Beijing Cancer Hospital, Beijing 100036, China 3. Department of Surgery ,Peking University Third Hospital, Beijing 100191, China 4. Department of Pathology ,Peking University Third Hospital, Beijing 100191, China 5. Department of Medical Oncology, Cancer Institute & Hospital Chinese Academy of Medical Sciences, Beijing 100021, China |
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Abstract: | Background Human epidermal growth factor 2 (HER2) is one of the most important prediction factors, but only 25%-30% of breast cancer patients HER2 are positive. It is unknown whether there are other molecular markers that could be used to predict prognosis and recurrence in HER2 negative patients.This study investigated correlations of cyclin A2 and HER2 levels with clinical outcomes in 281 patients with invasive breast cancer in order to identify whether cyclin A2 can serve as a prognostic factor in HER2 negative patients.Methods Immunohistochemical staining was used to detect cyclin A2 and HER2 expression in 281 patients. Cyclin A2. and HER2 gene amplifications were analyzed using gene analysis and RT-PCR in 12 patients. Risk and survival estimates were analyzed using Log-rank, Kaplan-Meier, and Cox regression analysis; cyclin A2 and HER2 consistency with survival were analyzed using Kappa analysis.Results Patients with higher cyclin A2 and HER2 expressions had significantly shorter disease-free survival periods (P=0.047 and P=0.05, respectively). Kappa analysis performed that cyclin A2 and HER2 showed a low Kappa index (kappa=0.37), allowing us to conclude that cyclin A2 and HER2 detect different pathologies. Gene analysis and RT-PCR showed that cyclin A2 was upregulated in patients with early relapse; the average increase was 3.69-2.74 fold.Conclusions Cyclin A2 and HER2 are associated with proliferation and high recurrence, particularly when combined. Cyclin A2 is easily detected by nuclear staining and might be a useful biomarker for recurrence risk in HER2 negative patients. |
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Keywords: | breast neoplasms cyclin A2 human epidermal growth factor 2 prognosis |
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