| Value of polymerase chain reaction assay in diagnosis of intestinal tuberculosis and differentiation from Crohn's disease. |
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| 作者姓名： | 甘华田 欧阳钦 步宏 李蜀华 陈德珍 李甘地 杨秀英 |
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| 作者单位： | Department of Gastroenterology. West China University of Medical Sciences. Chengdu 610041，Department of Gastroenterology. West China University of Medical Sciences. Chengdu 610041，Department of Pathology，the First Affiliated Hospital. West China University of Medical Sciences. Chengdu，Department of Pathology，the First Affiliated Hospital. West China University of Medical Sciences. Chengdu，Department of Gastroenterology. West China University of Medical Sciences. Chengdu 610041，Department of Pathology，the First Affiliated Hospital-West China University of Medical Sciences.，Department of Pathology，the First Affiliated Hospital-West China University of Medical Sciences. |
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| 摘 要： | It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intestinal tuberculosis from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were subjected to PCR assay. 21 specimens of normal colon tissue surrounding cancer were used as the control. Oligonucleotides derived from the IS 6110 sequence, which is repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The amplified PCR products were detected by examination of ethidium-bromide-stained polyacrylamide gels. The specificity of PCR products was confirmed by digestion with Sal 1 restrictive endonuclease and southern blot hybridization using digoxigenin-labeled probe. The results showed that the M. tuberculosis DNA was identified in 27 / 36 intestinal tuberculosis, but none of 26 Crohn's disease. Acid fast bacilli were o
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