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Tumor blood vessels formation in osteosarcoma: vasculogenesis mimicry
作者姓名:Cai XS  Jia YW  Mei J  Tang RY
作者单位:[2]DepartmentofOrthopedicsTongjiHospital,TongjiUniversity,Shanghai200065,China [3]DepartmentofPathology,TongjiHospital,TongjiUniversity,Shanghai200065,China
基金项目:ThisstudywassupportedinpartbyagrantfromtheNationalNaturalScienceFoundationofChina (No 3 0 2 713 14 )
摘    要:Background Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarooma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. Methods Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarooma were also performed. Results Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicel of osteochondroma or outside osteochondroma were endothelial-lined. Conclusion These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.

关 键 词:肿瘤血管生成  骨肉瘤  免疫组织化学  肿瘤生物学  血管内皮生长因子

Tumor blood vessels formation in osteosarcoma: vasculogenesis mimicry
Cai XS,Jia YW,Mei J,Tang RY.Tumor blood vessels formation in osteosarcoma: vasculogenesis mimicry[J].Chinese Medical Journal,2004,117(1):94-98.
Authors:Cai Xuan-song  Jia Yong-wei  Mei Jiong  Tang Ru-yong
Institution:1. Department of Orthopedics, Tongji Hospital, Tongji University, Shanghai 200065, China
2. Department of Pathology, Tongji Hospital, Tongji University, Shanghai 200065, China
Abstract:BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. METHODS: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarcoma were also performed. RESULTS: Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicle of osteochondroma or outside osteochondroma were endothelial-lined. CONCLUSION: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.
Keywords:osteosarcoma  vasculogenesis mimicry  angiogenesis  3-dimensional cultures  immunohistochemistry
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