夏枯草及联合化疗药物对Raji细胞增殖的影响

目的探讨夏枯草（prunella vulgaris，PV）及与化疗药物联合对人淋巴瘤细胞Raji体外生长的抑制作用。方法MTT法测定夏枯草及分剐与紫杉醇（TAX）、顺铂（DDP）、长春新碱（VCR）联合应用对Raji细胞的生长抑制率；免疫组化方法检测夏枯草对Raji细胞内Survivin及Caspase-3蛋白表达的影响。结果①夏枯草抑制Raji细胞的生长，其半数抑制浓度（IC50）为30．26μg／ml且呈剂量相关性。②夏枯草（10μg／ml）与TAX、DDP、VCR分别合用时抑制率较单用TAX、DDP时的抑制率高（P〈0．05），但较单用VCR时无明显增加抑制作用。③免疫组化结果显示30μg/ml夏枯草作用于Raji细胞48h后，Survivin表达减弱，而Caspase-3蛋白表达增强。结论夏枯草对Raji细胞增殖具有抑制作用，其抗肿瘤机制可能与诱导细胞凋亡有关；与TAX、DDP联合应用，可提高其化疗效果。

Effects of prunella vulgaris alone or combined with chemotherapeutic agents on proliferation of Raji cells

Objective To observe the effects of prunella vulgaris （PV） alone or combined with chemotherapeutic agents on proliferation of human lymphoma cell line Rail. Methods The growth inhibition rate of Raji cells treated with PV combined with TAX, DDP and VCR, were examined by MTT assay respectively. The expression of apoptosis related protein survivin, caspase -3 was measured by immu- nocytoehemistry. Results （1）PV could suppress the proliferation of Raji cells in a dose - dependent manner. The IC50 was 30.26 μg/ml. （2）The inhibitory rates of PV combined respectively with TAX, DDP and VCR were significantly higher than those of TAX, DDP alone （ P 〈 0.05 ） , but not significantly different from those with VCR alone. （3）The results of immunocytochemistry showed that after Raji ceils were treated by PV （30 μg/ml） for 48 hours, the expression of survivin was down - regulated, the expression of caspase - 3 was up - regulated. Conclusions PV can inhibit the .proliferation of Raji cells. The anti -lymphoma mechanisms may be correlated with inducing the apoptosis of ceils. The chemotherapy effect can be enhanced by the combination of PV with TAX, DDP.