| PBCA纳米粒介导反义寡核苷酸转染对肺腺癌细胞hTERT的影响 |
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| 引用本文： | 张艳艳,付旭东,黄幼田,张振中.PBCA纳米粒介导反义寡核苷酸转染对肺腺癌细胞hTERT的影响[J].中原医刊,2006,33(11):5-6. |
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| 作者姓名： | 张艳艳 付旭东 黄幼田 张振中 |
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| 作者单位： | 郑州大学基础医学院病理生理学教研室，郑州大学第一附属医院神经外科，郑州大学基础医学院病理生理学教研室，郑州大学药学院 郑州450052，郑州450052 |
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| 基金项目： | 国家自然科学基金资助项目（30371695） |
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| 摘 要： | 目的以聚氰基丙烯酸丁酯纳米粒(PBCA-NPs,NPs)作为端粒酶逆转录酶(hTERT)反义寡核苷酸(ASODN)载体转染肺腺癌A549细胞,观察其对该细胞hTERT mRNA表达的影响。方法以NPs携带ASODN转染A549细胞后,用流式细胞仪检测细胞周期;逆转录-聚合酶链反应(RT-PCR)检测细胞hTERT mRNA表达的改变。结果转染后,与空白对照组和正义寡核苷酸(SODN)组相比,ASODN组G0/G1期细胞增加,S期细胞明显减少(P<0.01),hTERT mRNA表达明显下降(P<0.01)。结论hTERT ASODN能有效改变A549细胞的细胞周期,下调hTERTmRNA表达,对肺腺癌细胞生长有抑制作用。
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| 关 键 词： | 端粒酶逆转录酶 反义寡核苷酸 A549细胞 基因表达 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006年4月10日 |
Influence of antisense oligodeoxynucleotide on hTERT by PBCA nanoparticles on A549 cell |
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| ZHANG Yanyan,HUANG Youtian,FU Xudong,ZHANG Zhenzhong..Influence of antisense oligodeoxynucleotide on hTERT by PBCA nanoparticles on A549 cell[J].Central Plains Medical Journal,2006,33(11):5-6. |
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| Authors: | ZHANG Yanyan HUANG Youtian FU Xudong ZHANG Zhenzhong |
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| Institution: | 1. Department of Pathophysiology, Basic Medical Sciences College, Zhengzhou University, Zhengzhou 450052, Henan ; 2. Department of Neurosurgery, the First Affiliated Hospital, Zhengzhou University; 3. Pharmaceutical College, Zhengzhou University |
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| Abstract: | Objective To investigate the effect of hTERT antisense oligodeoxynucleotide on the gene expression of hTERT mRNA by polybutylcyanoacrylate nanoparticles(PBCA-NPs,NPs).Methods After transfecting hTERT ASODN by NPs into A549 cell,cell cycles were analyzed by flow cytometry(FCM),the expression of hTERT mRNA was determined by RT-PCR.Results Compared with control group and(SODN-)NP group,the cell cycles in ASODN-NP group varied obviously and the cell cycle was blocked in G_0/G_1 phase obviously(P<0.01),the expression of hTERT mRNA of ASODN group decreased observably((P<)0.01).Conclusion hTERT ASODN mediated by PBCA-NPs can vary A549 cell cycles and reduce the expression of hTERT mRNA,it can inhibit the growth of A549 cell efficiently. |
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