牛磺酸对神经干细胞因子表达的影响

目的观察牛磺酸是否影响神经干细胞因子mRNA的表达。方法选择成年雄性SD大鼠63只，使用线栓法建立大脑中动脉缺血再灌注模型（MCAO）。随机分为治疗组（自造模当日开始，静脉注射80mg／kg牛磺酸，持续10min，每日1次，连用7d）和对照组（静脉注射0．9％氯化钠注射液1训，其余相同），每纽再随机分为再灌流2h、24h、3d、7d、14d组（n=6），假手术组（n=3）。原位杂交检测脑缺血再灌注后脑组织干细胞因子（stem cell factor，SCF）mRNA的表达。结果假手术组SCFmRNA在皮质、纹状体和室旁区有微弱的表达。对照组缺血侧SCFmRNA的表达，皮质除2h以外、纹状体除2h以外、室旁区除2h、14d以外各时间点均明显高于假手术组（t=2．16—25．19，P〈0．05）。治疗组SCFmRNA表达较对照组在皮质、纹状体及室旁区均显著升高（t：5．19～26．17，P〈0．05）。结论牛磺酸可以促进大鼠局灶性脑缺血再灌注后SCFmRNA的表达。

Effect of taurine on the expression of neural stem cell factor

Objective To observe the effect of taurine on the expression of neural stem cell factor mRNA. Methods Sixty - three adult male rats were randomly divided into taurine - treated group （ Taurine, 80 mg/kg, began at the day of MCAO and continuously intravenous injection for 10 rain, daily, for 7 days） and controlled group （0. 9% normal saline, 1 m], continuously intravenous injection for 10 min, daily, for 7 days）. The rats were received fight middle cerebral artery occlusion of 2 h and different hours of repeffusion. Expressions of SCF was detected by in situ hybridization in the rats subjected to 2 h, 24 h, 3 d, 7 d, 14 d of reperfusion （n = 6） and sham -operated group （n = 3）. Results Low level SCF mRNA expressions were found in cortex, striatum and extraventricular zone in sham -operated group. The expression of SCF mRNA in isehemie hemisphere of controlled group increased markedly compared with sham -operated group （t = 2. 16 -25.19, P 〈 0. 05）, except that of 2 h in cortex, 2 h in striatum, 2 h and 14 d in extraventrieular zone. SCF mRNA expression in the treatment group increased markedly in cortex, striatum and extraventrieular zone at 3 d and 7 d （t = 5. 19 - 26. 17,P 〈 0.05）. Conclusions Taurine increased the SCF mRNA expression in MCAO rats, therefore it might promote the expression of neural stem cell.