大鼠骨髓间充质干细胞向神经细胞分化过程中Notch信号与RhoA/Rho激酶信号的协同作用

目的 探讨Notch信号通路在大鼠骨髓间充质干细胞(MSCs)向神经细胞分化中与RhoA/Rho激酶信号的协同作用.方法 实验分为无血清培养基对照组和盐酸法舒地尔组.采用盐酸法舒地尔诱导大鼠MSCs分化为神经细胞.RT-PCR检测Hes1的表达变化;免疫细胞化学法检测神经元特异性烯醇化酶(NSE)、神经微丝蛋白亚单位(NF-M)和胶质纤维酸性蛋白(GFAP)的表达变化.结果 ①盐酸法舒地尔组MSCs的Hes1 mRNA转录下降(P<0.05).②盐酸法舒地尔可以诱导MSCs向神经细胞分化,NSE、NF-M的阳性表达率显著高于无血清培养基组(P<0.05).结论 盐酸法舒地尔在诱导大鼠MSCs向神经细胞分化过程中,可能存在Notch信号通路与RhoA/Rho激酶通路信号的协同作用,共同促进MSCs向神经细胞分化. Abstract： Objective To investigate the cross-talk between notch and Rho/Rho GTPase signaling on rat bone marrow mesenchymal stem cells differentiating into neurons. Methods The experiments were divided into serum-free medium control group and fasudil hydrochloride group. Fasudil hydrochloride induces MSCs differentiating into neurons. The expression of Hes1 mRNA was detected by RT-PCR. The expression of neuron-specific enolae(NSE), neurofilament M (NF-M) and glial fibrillary acidic protein (GFAP)was detected by immunocytochemical method. Results ①The Hes1 mRNA expressed by MSCs of fasudil hydrochloride group was significantly decreased(P<0.05).②Fasudil hydrochloride can induce MSCs differentiating into neurons and there was higher expression of neuron-specific enolae(NSE) and neurofilament-M (NF-M) than the serum-free medium control group(P<0.05).Conclusions There may be cross-talk between notch signaling and RhoA/Rho GTPase signaling on differentiation of rat bone marrow stromal cell into neurons induced by fasudil hydrochloride and they jointly promoted the differentiation of MSCs into neurons.

Cross-talk between notch signaling and RhoA/Rho GTPase signaling on rat bone marrow mesenchymal stem cells differentiating into neurons

Notch Rho Fasudil hydrochloride Marrow mesenchymal stem cells Neurons