| Ubc9对肺癌NCI-H446细胞凋亡的影响及机制探讨 |
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| 引用本文： | 李慧,吴宝刚,彭扬,白雪,何平.Ubc9对肺癌NCI-H446细胞凋亡的影响及机制探讨[J].中国全科医学,2011,14(33). |
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| 作者姓名： | 李慧 吴宝刚 彭扬 白雪 何平 |
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| 作者单位： | 中国医科大学附属盛京医院老年病科,辽宁省沈阳市,110004 |
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| 摘 要： | 目的探讨过表达Ubc9蛋白对人肺癌NCI-H446细胞凋亡的影响及可能机制。方法构建pEGFP-N1-Ubc9真核表达载体,稳定转染到肺癌细胞系NCI-H446细胞,以蛋白免疫印迹实验检测外源Ubc9的表达,以流式细胞术分析细胞凋亡情况,利用Western blot方法检测转染前后NCI-H446细胞Bcl-2蛋白表达水平。结果成功构建Ubc9真核表达质粒,蛋白免疫印迹实验证实外源性Ubc9蛋白质于肺癌NCI-H446细胞高表达,流式细胞术检测发现目的蛋白质过表达后的细胞凋亡比率降低,转染组凋亡率(5.52±0.48)%明显低于空转染组的(10.11±0.92)%,差异有统计学意义(P<0.05)。Western blot方法检测转染组Bcl-2蛋白表达水平则高于空转染组。结论Ubc9高表达可以抑制NCI-H446细胞的凋亡,其凋亡水平的改变与影响Bcl-2蛋白的表达有关。
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| 关 键 词： | 肺肿瘤 细胞凋亡 流式细胞术 |
Impact of Ubc9 on the Apoptosis of Lang Cancer NCI-H446 Cells and the Possible Mechanisms |
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| LI Hui,WU Bao-gang,PENG Yang,et al..Impact of Ubc9 on the Apoptosis of Lang Cancer NCI-H446 Cells and the Possible Mechanisms[J].Chinese General Practice,2011,14(33). |
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| Authors: | LI Hui WU Bao-gang PENG Yang |
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| Institution: | LI Hui,WU Bao-gang,PENG Yang,et al.Department of Geriatrics,Affiliated Shengjing Hospial of China Medical University,Shenyang 110004,China |
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| Abstract: | Objective To explore the influence and possible mechanisms of Ubc9 protein high expression on NCI-H446 cell apoptosis in human lung cancer.Methods We constructed a pEGFP-N1-Ubc9 eukaryotic expression vector,which was transfected stably into lung cancer NCI-H446 cells,with exogenous Ubc9 expression detected through protein immune imprinting experiment.We analyzed the apoptosis status of the cells by Flow CytoMeter and detected Bcl-2 protein expression before and after transfection using Western blot.Results ... |
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| Keywords: | Lung neoplasms Apoptosis Flow cytometry |
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