热毒宁抗呼吸道合胞病毒(RSV,Long株)作用体外实验研究

目的:研究中草药热毒宁注射液对呼吸道合胞病毒(RSV,Long株)的抑制作用.方法:以病毒唑为阳性对照药,采用细胞培养技术,观察不同药物浓度及不同给药方式下RSV攻击后各组Hep-2细胞的病变效应(CPE),在此基础上采用MTT比色法,测定各组细胞的病毒抑制率.以CPE法计算药物的半数中毒浓度TC50,分别以CPE法及MTT法计算药物的半数有效浓度EC50及治疗指数TI,比较不同药物浓度及不同给药方式下热毒宁对呼吸道合胞病毒(RSV)的抑制作用效果.结果:热毒宁注射液半数中毒浓度TC50为3.972g/L.预防给药方式、细胞外直接灭活及治疗给药方式均有抗RSV作用,其抗RSV的半数有效浓度(EC50)分别为0.428、1.160、1.189 g/L,治疗指数TI分别为9.28、3.42和 3.34,热毒宁对RSV的抑制作用存在着明显的量效反应关系.结论:热毒宁对RSV有直接灭活作用,对RSV侵入Hep-2细胞有阻断作用,对RSV在Hep-2细胞内增殖有抑制作用,相同浓度下,以预防作用更显著.

Anti-respiratory syncytial virus(RSV,Long strain) effect of Reduning inject on in vitro

Objective:To explore anti-reispiratory syncytial virus(RSV) effect of Chinese medicinal Reduning injection in vitro. Method:The technique of cell culture and MTT with ribavirin as positive control to observe anti-reispiratory syncytial virus effect. Results:This drug was found to be a potential inhibitor of RSV in a concentration-dependent manner with the median toxic concentration(TC50) of 3.972 g/L in Hep-2 Cell culture. The median effective concentration(EC50) was 0.428 g/L-1 when drug was added 24 h before infection. The EC50 was 1.160 g/L when drug was added 2 h after virus infection,and it was 1.189 g/L as the drug was added after it mixed with virus 24 h. The therapeutic index(TI) was 9.28,3.42 and 3.34 respectively. Conclusion:Reduning injection has the direct deactivation and blocking effect on RSV. The preventive effect is the most evident in three drug deliveries drug with the same concentration in virto.