抑癌基因WWOX 和FHIT在结直肠癌中的表达及其临床意义

目的:观察抑癌基因-包含WW域的氧化还原酶(WWOX)和脆性组氨酸三联体(FHIT)在结直肠癌和癌旁正常大肠组织中的表达水平及与临床病理特征、预后之间的关系并探讨两者表达是否具有相关性.方法:应用免疫组织化学PV-9000通用型二步法检测75例结直肠癌和30例癌旁正常大肠组织中WWOX和FHIT、基因的蛋白表达.结果:在结直肠癌组织中WWOX和FHIT蛋白阳性表达率明显低于癌旁正常大肠组织(P＜0.05),且这种表达状态与组织学分级、Dukes分期及5年生存率密切相关(P＜0.05).而与部位和年龄无显著相关.WWOX和FHIT蛋白表达之间有相关性,这种相关性可能与常见脆性位点对DNA损伤的易感性相一致有关.结论:WWOX和FHIT基因在结直肠癌的发生、发展过程中可能具有较为重要的作用,并可能联合预测结直肠癌的预后.

The expression and clinical significance of WWOX and FHIT in colorectal cancer

Objective:To explore the expression and significance of WWOX and FHIT in colorectal cancer.Methods:The expression of WWOX and FHIT was evaluated by immunohistochemical staining(two-step method)in 75 colorectal carcinoma specimens and 30 normal paraneoplastic colorectal tissues,and their correlation with clinicopathological features was analyzed. Results:The rate of WWOX and FHIT positive expression in tumors was 52% and 54.7%,respectively,which was significantly different from that in normal paraneoplastic colorectal tissues as 93.3% and 97.8%.WWOX and FHIT expression were correlated strongly(P<0.05).The expression of WWOX and FHIT was closely associated with histological grade,Duke stage and 5-year survival rate.And the expression of wwox and FHIT had no significant correlation with neoplastic site and ages.There was a correlation between WWOX expression and FHIT This correlation was consistent with the susceptibility of common fragile sites to DNA damage.Conclusion:The positive expression of WWOX and FHIT was lower than the normal tissues and closely associated with histological grade,Duke stage,and 5-year survival rate(P<0.05).WWOX and FHIT expression were correlated strongly(P<0.05). It is suggested that WWOX and FHIT genes may play an important role in the initiation and development of colorectal cancer.They can become targets of predicting the progression of colorectal cancer.